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CSMD1 Antibody

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Western Blot: CSMD1 Antibody [NBP2-29341] - (1ug/ml) Analysis of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: CSMD1 Antibody [NBP2-29341] - (1ug/ml) Analysis of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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CSMD1 Antibody Summary

Immunogen
Peptide with sequence C-KGVREVNETVTKTP corresponding to internal region according to NP_150094.4.
Predicted Species
Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CSMD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 150 kDa band observed in human brain is reported in scientific literature (PMID: 16547280).
Publications
Read Publication using NBP2-29341.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CSMD1 Antibody

  • CSMD1
  • CSMD-1
  • CUB and sushi domain-containing protein 1
  • CUB and sushi multiple domains protein 1
  • KIAA1890
  • Mdcr1
  • PPP1R24
  • UNQ5952/PRO19863

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: CSK Antibody [NBP1-85951] - Analysis in human cell line RT-4 and human cell line HeLa.
Western Blot: CSK Antibody [NBP1-85951] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
(1 Publication)
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Applications: ICC/IF, IHC, IHC-P, WB
CSMD3 Antibody
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Immunohistochemistry-Paraffin: CSMD3 Antibody [NBP1-86371] - Staining of human testis shows moderate membranous positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: CSMD3 Antibody [NBP1-86371] - Staining of human prostate shows moderate membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: CSMD3 Antibody [NBP1-86371] - Staining of human Fallopian tube shows moderate membranous positivity in glandular cells.
Species: Hu
Applications: IHC, IHC-P
CSMD2 Antibody
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Immunohistochemistry-Paraffin: CSMD2 Antibody [NBP1-93569] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.
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Tmp21/p23 Antibody
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Knockdown Validated: Tmp21/p23 Antibody [NB110-57586] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TMP21/TMED10 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human TMP21/TMED10 Polyclonal Antibody (Catalog # NB110-57586) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for TMP21/TMED10 at approximately 25 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.
Western Blot: Tmp21/p23 Antibody [NB110-57586] - TMP21 Antibody [NB110-57586] - Detection of TMP21 in human liver lysate.
Immunohistochemistry: Tmp21/p23 Antibody [NB110-57586] - TMP21 Antibody [NB110-57586] - Detection of TMP21 in central neurons.
Species: Bv, Ch, Hu, Mu, Pm, Rb, Rt
Applications: IHC, IHC-P, KD, WB
Connexin 26/GJB2
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Western Blot: Connexin 26/GJB2 Antibody [NBP2-41304] - Analysis of Connexin 26/GJB2 in human colon tissue lysate with Connexin 26/GJB2 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/Immunofluorescence: Connexin 26/GJB2 Antibody [NBP2-41304] - Cx26 distribution at level of the cardiomyocyte cytoplasm. Confocal laser scanning microscopy: representative images of rat and pig heart sections. Double immunofluorescence analysis for Cx26 (red) and Cx43 (green) show a distinct cell localization of the two Cxs. (a) Three dimensional picture of the maximum intensity projection of the raw images of longitudinal rat heart section treated with R(alpha)Cx26-cl and M(alpha)Cx43. Scale bar: 20um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY licence.
Immunohistochemistry: Connexin 26/GJB2 Antibody [NBP2-41304] - Positive and negative tissue controls for Cx26. Representative images of immunohistochemical analysis for Cx26 on rat liver and skeletal muscle sections by R(alpha)Cx26-cl antibody. Cx26 positive brown punctate staining of hepatocyte plasma membrane (arrows) and cytoplasm (arrowheads) is shown in liver. By contrast, Cx26 staining is absent in skeletal muscle. Negative controls are showen in the squares. Scale bars: 50um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-32405-2), licensed under a CC-BY licence.
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Species: Hu, Po, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
MCPH1 Antibody
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, CHP-100 human neuroblastoma cell line, PC-12 rat adrenal pheochromocytoma cell line, Rat-2 rat embryonic fibroblast cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human MCPH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3998) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MCPH1 at approximately 105 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
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SLC6A4/5-HTTLPR/Seroto ...
H00006532-D01P
Flow Cytometry: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [H00006532-D01P] - Fixed and permeabilized mouse mast cells were stained for 1 hour in 4C then stained with PE secondary and ran on FACS. Image from verified customer review.
Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [H00006532-D01P] - Analysis of SLC6A4 expression in transfected 293T cell line by SLC6A4 polyclonal antibody. Lane 1: SLC6A4 transfected lysate (70.30 KDa). Lane 2: Non-transfected lysate.
Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [H00006532-D01P] - Analysis of SLC6A4 expression in mouse lung.
(1 Review)
(1 Publication)
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Immunohistochemistry: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863] - Characterization of TLC cultured in chondrogenic pellets. Representative images of histological and immunohistochemical staining of TLC pellets (CH medium) for COL2, SOX9, COL10, RUNX2, MKX, TNAP, and ENPP1. COL2 positive control was obtained with a chondrosarcoma sample. HE: Hematoxylin and eosin. Scale bar 100 um. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2077-0383/8/10/1544), licensed under a CC-BY licence.
Western Blot: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mohawk homeobox.
Immunohistochemistry-Paraffin: Mohawk homeobox Antibody (OTI6B12) [NBP2-45863] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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(1 Publication)
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Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p19ARF/CDKN2A in (A) NIH/3T3 and (B) MEF cells.
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(1 Review)
(45 Publications)
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Arylsulfatase
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Immunohistochemistry-Paraffin: Arylsulfatase G/ARSG Antibody [NBP1-82947] - Staining of human colon, liver, lymph node and pancreas using Anti-ARSG antibody NBP1-82947 (A) shows similar protein distribution across tissues to independent antibody NBP1-82948 (B).
Immunohistochemistry-Paraffin: Arylsulfatase G/ARSG Antibody [NBP1-82947] - Staining of human colon.
Immunohistochemistry-Paraffin: Arylsulfatase G/ARSG Antibody [NBP1-82947] - Staining of human liver.
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Applications: IHC, IHC-P
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H00006925-M03
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Immunocytochemistry/Immunofluorescence: TCF4 Antibody (1G4) [H00006925-M03] - Analysis of monoclonal antibody to TCF4 on HeLa cell. Antibody concentration 10 ug/ml
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(1 Review)
(6 Publications)
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Western Blot: GARS Antibody [NBP1-85533] - Analysis using Anti-GARS antibody NBP1-85533 (A) shows similar pattern to independent antibody NBP1-85534 (B).
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Applications: ICC/IF, IHC, IHC-P, WB
TCF7L2 Antibody
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Immunocytochemistry/Immunofluorescence: TCF7L2 Antibody (SC06-90) [NBP2-67618] - Staining TCF7L2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: TCF7L2 Antibody (SC06-90) [NBP2-67618] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-TCF7L2 antibody. Counter stained with hematoxylin.
(1 Publication)
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PKC theta Antibody
(453416) [Unconjugate ...
MAB4368
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PKC theta at approximately 82 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human PKC theta Monoclonal Antibody (Catalog # MAB4368). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Human PKC theta Monoclonal Antibody (Catalog # MAB4368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PKC theta at approximately 82 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Jurkat human acute T cell leukemia cell line was stained with Human PKC theta Monoclonal Antibody (Catalog # MAB4368, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
(2 Reviews)
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Applications: CyTOF-ready, ICFlow, Simple Western, WB
TEF1 Antibody
NBP2-94035
Chromatin Immunoprecipitation: TEF1 Antibody [NBP2-94035] - Analysis of extracts of HeLa cell line, using TEF1 rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Immunocytochemistry/Immunofluorescence: TEF1 Antibody [NBP2-94035] - Analysis of MCF7 cells using TEF1 .
Western Blot: TEF1 Antibody [NBP2-94035] - Analysis of extracts of various cell lines, using TEAD1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.
Species: Hu, Mu, Rt
Applications: ChIP, ICC/IF, IHC, IHC-P, WB
NKp30/NCR3
[Unconjugated]
1849-NK
NKp30/NCR3 [Unconjugated]
(18 Publications)
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Applications: BA
CDK5 Activator 1
Antibody
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Immunocytochemistry/Immunofluorescence: CDK5 Activator 1 Antibody [NBP3-13279] - CDK5 Activator 1 antibody detects CDK5 Activator 1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDK5 Activator 1 stained by CDK5 Activator 1 antibody (NBP3-13279) diluted at 1:500. Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: CDK5 Activator 1 Antibody [NBP3-13279] - CDK5 Activator 1 antibody detects CDK5 Activator 1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CDK5 Activator 1 stained by CDK5 Activator 1 antibody (NBP3-13279) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: CDK5 Activator 1 Antibody [NBP3-13279] - CDK5 Activator 1 antibody detects CDK5 Activator 1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. CDK5 Activator 1 stained by CDK5 Activator 1 antibody (NBP3-13279) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
c-Myc Antibody
(9E10) - BSA Free
NB600-302
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1003277) licensed under a CC-BY licence.
(4 Reviews)
(38 Publications)
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
Smad1 Antibody
[Unconjugated]
AF2039
Smad1 was detected in immersion fixed paraffin-embedded sections of human breast array using Goat Anti-Human Smad1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2039) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MBA‑MB‑468 human breast cancer cell line, and HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Smad1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2039) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Smad1 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and MDA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Smad1 at approximately 66 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Smad1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2039) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
(1 Review)
(4 Publications)
Species: Hu
Applications: IHC, Simple Western, WB
CSMD1 Antibody
NBP2-29341
CSMD1 Antibody
Species: Hu, Mu, Rt, Bv
Applications: WB, PEP-ELISA

Publications for CSMD1 Antibody (NBP2-29341)(1)


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CSMD1
Entrez