CSK Antibody (3A3) - Azide and BSA Free

Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK expression in transfected 293T cell line by CSK monoclonal antibody (M01), clone 3A3.Lane 1: CSK transfected lysate(50.7 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: CSK Antibody (3A3) [H00001445-M01] - Analysis of monoclonal antibody to CSK on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.

Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK over-expressed 293 cell line, cotransfected with CSK Validated Chimera RNAi ( Cat # H00001445-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CSK monoclonal antibody (M01) clone 3A3 (Cat # H00001445-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: CSK Antibody (3A3) [H00001445-M01] - CSK monoclonal antibody (M01), clone 3A3 Analysis of CSK expression in HL-60.

Western Blot: CSK Antibody (3A3) [H00001445-M01] - CSK monoclonal antibody (M01), clone 3A3. Analysis of CSK expression in Hela S3 NE.

Western Blot: CSK Antibody (3A3) [H00001445-M01] - Analysis of CSK transfected lysate using anti-CSK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CSK MaxPab rabbit polyclonal antibody.

ELISA: CSK Antibody (3A3) [H00001445-M01] - Detection limit for recombinant GST tagged CSK is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, IP, Mycoplasma
• Clone: 3A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CSK Antibody (3A3) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CSK (NP_004374, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSAIQAAWPSGTECIAKYNFHGTAEQDLPFCKGDVLTIVAVTKDPNWYKAKNKVGREGIIPANYVQKREGVKAGTKLSLMPWFHGKITREQAERLLYPPE
• Specificity: CSK - c-src tyrosine kinase
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSK
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CSK Antibody (3A3) - Azide and BSA Free

• CSK
• C-Src kinase
• c-src tyrosine kinase
• EC 2.7.10
• EC 2.7.10.2
• MGC117393
• Protein-tyrosine kinase CYL
• tyrosine-protein kinase CSK

Background

Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on theLYN and FYN kinases

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CSK Antibody (H00001445-M01)(1)

Publication using H00001445-M01

• Ge Y, Onengut-Gumuscu S, Quinlan AR et al. Targeted Deep Sequencing in Multiple-Affected Sibships of European Ancestry Identifies Rare Deleterious Variants in PTPN22 That Confer Risk for Type 1 Diabetes. Diabetes 2016-03-01 [PMID: 26631741]

Bioinformatics

• Gene Symbol: CSK
• Entrez:
  • 1445(Human)
• OMIM:
  • 124095(Human)
• Uniprot:
  • NP_004374(Human)