CRMP2 Antibody (33CT11.40.3)

Western Blot: CRMP2 Antibody (33CT11.40.3) [NBP1-50580] - Probenecid treatment decreases Crmp2 co-precipitation with Panx1. Western blot of a GFP IP from N2a cells expressing Panx1-EGFP, or EGFP control, treated (16 h) with probenecid or vehicle control. (ii) Probenecid treatment (16 h) significantly decreased the amount of Crmp2 that precipitated with Panx1-EGFP. Crmp2 signal in the IP fraction was normalized to GFP signal intensity from the same lane (Panx1-EGFP [vehicle] vs. Panx1-EGFP [probenecid]: 100.0 +- 0.8 vs. 39.4 +- 19.6, p = 0.0368, n = 3, unpaired t-test). Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2018.00124/full) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: CRMP2 Antibody (33CT11.40.3) [NBP1-50580] - Analysis of anti-CRMP-2 antibody with human kidney at dilution 1:200.

Western Blot: CRMP2 Antibody (33CT11.40.3) [NBP1-50580] - Mouse NIH/3T3 cell line, diluted at 1:2000. Goat anti-mouse at 1:3000 dilution was used as the secondary antibody. Lysate at 20ug.

Immunohistochemistry-Paraffin: CRMP2 Antibody (33CT11.40.3) [NBP1-50580] - Analysis of anti-CRMP-2 antibody with human testis at dilution 1:200.

Product Discontinued

CRMP2 Antibody (33CT11.40.3) Summary

• Immunogen: KLH-conjugated synthetic peptide between aa 463-492 from human CRMP2.
• Predicted Species: Rat (90%), Chicken (90%), Bovine (90%). Backed by our 100% Guarantee.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DPYSL2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200
  • Proximity Ligation Assay
  • Western Blot 1:2000
• Application Notes: Use in Proximity Ligation Assay reported in scientific literature (PMID: 29867357).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.9% Sodium Azide
• Purity: Protein G purified

Alternate Names for CRMP2 Antibody (33CT11.40.3)

• Collapsin response mediator protein 2
• CRMP-2
• CRMP2N2A3
• DHPRP2
• dihydropyrimidinase-like 2 long form
• dihydropyrimidinase-like 2
• dihydropyrimidinase-related protein 2
• DRP2collapsin response mediator protein hCRMP-2
• DRP-2ULIP-2
• ULIP2
• Unc-33-like phosphoprotein 2

Background

Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins involved in neuronal differentiation and axonal guidance. CRMP2 was previously shown to mediate the repulsive effect of Sema3A on axons and to participate in axonal specification. The CRMPs appear to play a complex role in axon growth as well as microtubule dynamics and axon induction. CRMPs localize to the lamellipodia and filopodia of axonal growth cones, suggesting a role in axon guidance. Moreover, CRMP2 is upregulated after axotomy, and appears to increase the formation of axon-type processes from hippocampal neurons. CRMP2 has been reported to bind tubulin dimers directly and modulate microtubule assembly. CRMPs have also been implicated in the pathogenesis of a paraneoplastic neurologic syndrome. Interaction studies have implicated phospholipase D2 (PLD2), the cytosolic tyrosine kinase Fes, and intersectin in CRMP function. Hyperphosphorylation of CRMP2 is an early event in the progression of Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRMP2 Antibody (NBP1-50580)(2)

Publications using NBP1-50580

• Xu X, Wicki-Stordeur LE, Sanchez-Arias JC et al. Probenecid Disrupts a Novel Pannexin 1-Collapsin Response Mediator Protein 2 Interaction and Increases Microtubule Stability Front Cell Neurosci 2018-05-11 [PMID: 29867357] (PLA, WB, Mouse)
• Lee M, Cho H, Jung SH et al. Circulating MicroRNA Expression Levels Associated With Internet Gaming Disorder Front Psychiatry 2018-03-12 [PMID: 29593587] (WB, Human)

Bioinformatics

• Gene Symbol: DPYSL2
• Entrez:
  • 1808(Human)
• Uniprot:
  • Q16555(Human)