CRISP-1 Antibody [DyLight 488]

Name: CRISP-1 Antibody [DyLight 488]
Brand: Novus Biologicals
SKU: AF4675K
Price: 569 USD
Availability: InStock

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Product Details

Summary

Reactivity	MuSpecies Glossary
Applications	WB, IHC
Clonality	Polyclonal
Host	Goat
Conjugate	DyLight 488

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Allophycocyanin AF4675A

Biotin AF4675B

CoraFluor 1 AF4675CL1

DyLight 350 AF4675D

DyLight 405 AF4675E

DyLight 550 AF4675L

DyLight 594 AF4675M

DyLight 650 AF4675W

DyLight 680 AF4675Y

DyLight 755 AF4675Z

FITC AF4675F

HRP AF4675H

Janelia Fluor 525 AF4675JF525

Janelia Fluor 549 AF4675I

Janelia Fluor 646 AF4675J

Janelia Fluor 669 AF4675JF669

PE AF4675P

PerCP AF4675C

Unconjugated AF4675

mFluor Violet 450 SE AF4675MFV450

mFluor Violet 500 SE AF4675MFV500

mFluor Violet 610 SE AF4675MFV610

View Available Formulations

Catalog# & Formulation	Size	Price
Standard Formulation AF4675

Carrier Free AF4675

Carrier Free AF4675-SP

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Conjugate	Catalog #	Availability	Size	Price
Allophycocyanin	AF4675A
Biotin	AF4675B
CoraFluor 1	AF4675CL1
DyLight 350	AF4675D
DyLight 405	AF4675E
DyLight 550	AF4675L
DyLight 594	AF4675M
DyLight 650	AF4675W
DyLight 680	AF4675Y
DyLight 755	AF4675Z
FITC	AF4675F
HRP	AF4675H
Janelia Fluor 525	AF4675JF525
Janelia Fluor 549	AF4675I
Janelia Fluor 646	AF4675J
Janelia Fluor 669	AF4675JF669
PE	AF4675P
PerCP	AF4675C
Unconjugated	AF4675
mFluor Violet 450 SE	AF4675MFV450
mFluor Violet 500 SE	AF4675MFV500
mFluor Violet 610 SE	AF4675MFV610

CRISP-1 Antibody [DyLight 488] Summary

Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse CRISP-1 Gln20-His244 Accession # Q03401
Specificity	Detects mouse CRISP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse CRISP-3 is observed.
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	CRISP1
Purity	Antigen Affinity-purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions	Immunohistochemistry Western Blot
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide
Purity	Antigen Affinity-purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for CRISP-1 Antibody [DyLight 488]

Acidic epididymal glycoprotein homolog
acidic epididymal glycoprotein-like 1
AEGL1
AEG-related protein
ARPAEG-like protein
CRISP1
CRISP-1
CRISP-1AEGL1HSCRISP1D
cysteine-rich secretory protein 1
cysteine-rich secretory protein-1 delta
HSCRISP1G
HUMARP

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CRISP-1 Antibody
[DyLight 488]

AF4675K

Species: Mu

Applications: WB, IHC

Publications for CRISP-1 Antibody (AF4675K) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

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Loss of miR-101 promotes cancer metastasis through de-repression of COUP-TFII expression.(a) LNCaP and PC3 cells were individually treated with 50 nM of miR-101 and miR-27a mimic for 48 h and then invasion assays were performed for an additional 16 h (n=3). *P1.5-fold are represented as a heatmap. The gene symbol of FOXM1 and CENPF genes after knockdown of COUP-TFII are annotated in red colour. The others were FOXM1 and CENPF co-regulated genes annotated in black colour. siCtrl: knockdown scramble control; siCII: knockdown of COUP-TFII. (b) A representative western blot shows the level of COUP-TFII in PC3 cells transfected with two different siRNAs against COUP-TFII for 72 h (upper panel). Levels of FOXM1 and CENPF are quantified by RT-qPCR (lower panel) (n=3). *P<0.05 (two-sided Student's t-test) compared with the control group (siCON). (c) A cartoon shows the FOXM1 and CENPF loci. COUP-TFII-binding sites are annotated by a black rectangle (upper panel). ChIP results are shown in the PC3 cells treated with control and two different siRNAs against COUP-TFII for 72 h (lower panel) (n=3). *P<0.05 (two-sided Student's t-test) compared with control. (d) FOXM1 and CENPF promoter activities were measured in PC3 cells carrying an inducible COUP-TFII gene in the absence or the presence of doxycycline for 48 h (n=3). *P<0.05 (two-sided Student's t-test). (e) FOXM1 and CENPF promoter activities were measured in control or stably knocked-down COUP-TFII in PC3 cells for 48 h (n=3). *P<0.05 (two-sided Student's t-test). (f) PC3 cells carrying with inducible COUP-TFII-Flag gene were treated with or without doxycycline for 48 h and then transfected with individual siRNA against FOXM1 and CENPF or double knockdown of FOXM1 and CENPF for an additional 48 h. western blot (left panel) shows the level of COUP-TFII, CENPF and FOXM1 expression. Invasion assay (right panel) was performed by using those cells (n=3). *P<0.05 (two-sided Student's t-test) compared with control. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms11418), licensed under a CC-BY license. Not internally tested by R&D Systems.

COUP-TF II/NR2F2 Antibody (H7147) [Unconjugated]
PP-H7147-00

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Bioinformatics

Gene Symbol

CRISP1

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

CRISP-1 Antibody [DyLight 488]

Species Glossary

Images

Product Details

Order Details

CRISP-1 Antibody [DyLight 488] Summary

Applications/Dilutions

Packaging, Storage & Formulations

Notes

Alternate Names for CRISP-1 Antibody [DyLight 488]

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for CRISP-1 Antibody (AF4675K) (0)

Reviews for CRISP-1 Antibody (AF4675K) (0)

Product General Protocols

Video Protocols

FAQs for CRISP-1 Antibody (AF4675K) (0)

Secondary Antibodies

Isotype Controls

Additional CRISP-1 Products

Blogs on CRISP-1

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics