CRIPT Antibody

Western Blot: CRIPT Antibody [NB120-16422] - Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-CRIPT )

Product Discontinued

CRIPT Antibody Summary

• Immunogen: Recombinant fragment, corresponding to amino acids 1-101 of Human CRIPT.
• Specificity: CRIPT
• Clonality: Polyclonal
• Host: Chicken
• Gene: CRIPT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: WB: 1/500. Predicted molecular weight: 11 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for CRIPT Antibody

• Cysteine-rich interactor of PDZ three
• Cysteine-rich interactor of PDZ3
• cysteine-rich PDZ-binding protein
• HSPC139
• postsynaptic protein CRIPT

Background

The postsynaptic density (PSD) of excitatory synapses is a specialized structure in which an array of synaptic membrane proteins is physically linked and functionally coupled to a specific set of cytoskeletal and/or signaling proteins. The synaptic protein PSD95 binds to and clusters a variety of membrane proteins, including Shaker-type potassium channel subunits and NMDA receptor NR2 subunits, via its first 2 N-terminal PDZ domains. This clustering function implies a direct or indirect interaction with the cytoskeleton. CRIPT (cysteine-rich interactor of PDZ3) binds selectively to the third PDZ (PDZ3) domain of PSD95 via its C terminus. Human CRIPT is a 101-amino acid protein that terminates in a glu-thr-ser-val sequence, consistent with the PDZ-binding tetrapeptide consensus. It shares 99% amino acid sequence identity with rat CRIPT. CRIPT colocalizes with PSD95 in the PSD of excitatory synapses throughout the brain, but is not detected in inhibitory synapses. CRIPT, PSD95, and tubulin may be coimmunoprecipitated from synaptosomal membranes. In heterologous cells, CRIPT caused a redistribution of PSD95 to microtubules. Niethammer et al. (1998) suggested that CRIPT may regulate PSD95 interaction with a tubulin-based cytoskeleton in excitatory synapses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRIPT
• Entrez:
  • 9419(Human)
  • 56724(Mouse)
  • 56725(Rat)