Recombinant Human CRIF1 His Protein

SDS-Page: Recombinant Human CRIF1 Protein [NBP1-72519] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human CRIF1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 48-222 of Human CRIF1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPRWQLGPRY AAKQFARYGA ASGVVPGSLW PSPEQLRELE AEEREWYPSL ATMQESLRVK QLAEEQKRRE REQHIAECMA KMPQMIVNWQ QQQRENWEKA QADKERRARL QAEAQELLGY QVDPRSARFQ ELLQDLEKKE RKRLKEEKQK RKKEARAAAL AAAVAQDPAA SGAPSS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GADD45GIP1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 22.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 40% glycerol, 2 mM DTT, 0.2 M NaCl
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human CRIF1 His Protein

• CKBBP2
• CKII beta-associating protein
• CR6 interacting factor 1
• CRIF1p53-responsive gene 6 protein
• growth arrest and DNA damage-inducible proteins-interacting protein 1
• growth arrest and DNA-damage-inducible, gamma interacting protein 1
• MGC4667
• MGC4758
• papillomavirus L2 interacting nuclear protein 1
• Papillomavirus L2-interacting nuclear protein 1
• Plinp1
• PLINP-1CKII beta binding protein 2
• PRG6CR6-interacting factor 1

Background

GADD45GIP1, also known as CRIF1, is a nuclear protein that plays a role in apoptosis control. This protein is expressed in a variety of tissues, including heart, thyroid, trachea, kidney, ovary, pancreas, testis and stomach. It functions as a negative regulator of G1 to S phase cell cycle production, specifically by working with GADD45 proteins to inhibit the activity of cyclin-dependent kinases. Recombinant human GADD45GIP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GADD45GIP1