CRHR2/CRF2 Antibody

Western Blot: CRHR2/CRF2 Antibody [NBP3-03059] - Analysis of extracts of various cell lines, using CRHR2/CRF2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit

Product Discontinued

CRHR2/CRF2 Antibody Summary

• Immunogen: A synthetic peptide of human CRHR2/CRF2
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRHR2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 50% glycerol, pH7.3.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CRHR2/CRF2 Antibody

• corticotropin releasing hormone receptor 2
• corticotropin-releasing factor receptor 2
• Corticotropin-releasing hormone receptor 2
• CRF2
• CRF2R
• CRFR2
• CRF-R2
• CRF-R-2
• CRFR-2
• CRF-RB
• CRH2R
• CRHR2
• CRH-R2
• CRH-R-2
• HM-CRF

Background

Official Gene Symbol: CRHR2 Gen Bank Accession Number: NP_001874 Gene ID: 1395 (Human) Gene Map Locus: 7p15.1 (Human) CRHR2 is a novel member of Secretin-family-GPCR proteins that functions as a functional receptor for CRF. It is expressed as 3 isoforms; alpha, beta and gamma. These isoforms primarily differ in their N-terminal sequence and tissue distribution. Apart from CRF, other ligands of CRHR2 include UcnI, UcnII and UcnIII. CRHR2 is involved in stress responses, cardiovascular function and gastric motility. Studies on CRHR2 deficient mice suggested a central anxiolytic effect of CRHR2. Northern Blot analysis detects CRHR2 expression in brain, hypothalamus, heart, GI, lung, skeletal muscle and vasculature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRHR2