CRHR2/CRF2 Antibody

Product Discontinued

CRHR2/CRF2 Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 95-132 of Human CRF-RII.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRHR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of Corticotropin Releasing Factor Receptor 2 (Y113) pAb in extracts from COLO205 cells. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 29163012).
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CRHR2/CRF2 Antibody

• corticotropin releasing hormone receptor 2
• corticotropin-releasing factor receptor 2
• Corticotropin-releasing hormone receptor 2
• CRF2
• CRF2R
• CRFR2
• CRF-R2
• CRF-R-2
• CRFR-2
• CRF-RB
• CRH2R
• CRHR2
• CRH-R2
• CRH-R-2
• HM-CRF

Background

Individuals suffering from Alzheimer's disease (AD) exhibit dramatic reductions in the content of corticotropin-releasing factor (CRF), increased expression of CRF receptors (CRFRs) and abnormalities in neuronal morphology in affected brain areas. In addition, AD patients show decreased concentrations of CRF in their cerebrospinal fluid, which may contribute to their cognitive impairment. A high affinity CRF binding protein, designated CRF-BP, has been discovered in postmortem brain samples from AD patients. CRF-BP serves to bind and inactivate CRF, reducing the pool of free CRF available to bind CRFRs. Two CRF receptors, designated CRF-RI and CFR-RII, exhibit distinct brain localizations.Two forms of CFR-RII, designated CFR-RIIalpha and CFR-RIIbeta, result from alternative mRNA splicing. Urocortin, an additional member of the CRF family, shares 63% sequence identity with urotensin and 45% sequence identity with CRF. Urocortin specifically binds to and activates CRF-RI and CRF-RII, but binds to CRF-RII more efficiently than CRF, suggesting that it may be the true, high affinity ligand for the CRF receptor type II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRHR2/CRF2 Antibody (NBP1-00768)(2)

Publications using NBP1-00768

• Vinzant N, Scholl JL, Wu CM et al. Iron Oxide Nanoparticle Delivery of Peptides to the Brain: Reversal of Anxiety during Drug Withdrawal Front Neurosci. 2017-11-01 [PMID: 29163012] (IHC-Fr, Rat)
• Lemos JC, Wanat MJ, Smith JS et al. Severe stress switches CRF action in the nucleus accumbens from appetitive to aversive Nature 2012-09-19 [PMID: 22992525] (ICC/IF, IF/IHC, Mouse)

Reviews for CRHR2/CRF2 Antibody (NBP1-00768) (2)

Average Rating: 3

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Kasia Szymanska WB Human 10/30/2013

Summary

• Application: Western Blot
• Sample Tested: IMCD3, hTertRPE1, HDF, SHSY5Y whole cell lysates
• Species: Human
• Lot: 360980

reviewed by: Gabrielle Wheway IF Mouse 10/14/2013

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse inner medullary collecting duct (IMCD3) cell line
• Species: Mouse
• Lot: 360969

Bioinformatics

• Gene Symbol: CRHR2
• Entrez:
  • 1395(Human)
  • 64680(Rat)