CRHR1/CRF1 Antibody

Images

 
Western Blot: CRHR1/CRF1 Antibody [NBP2-16010] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary ...read more
Immunohistochemistry-Paraffin: CRHR1/CRF1 Antibody [NBP2-16010] - Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Rb, RMSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CRHR1/CRF1 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CRFR1. The exact sequence is proprietary.
Localization
Cell membrane
Predicted Species
Rat (100%), Rabbit (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CRHR1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-16010 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CRHR1/CRF1 Antibody

  • CHR-R1
  • corticotropin releasing hormone receptor 1
  • corticotropin-releasing factor receptor 1
  • corticotropin-releasing factor type 1 receptor
  • Corticotropin-releasing hormone receptor 1
  • CRF1
  • CRFR
  • CRF-R
  • CRFR1
  • CRF-R1
  • CRF-R-1
  • CRFR-1
  • CRHR1
  • CRH-R-1
  • CRHR1f
  • CRH-R1h
  • CRHR1L
  • CRHRCRH-R1
  • seven transmembrane helix receptor

Background

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for CRHR1/CRF1 Antibody (NBP2-16010) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-16010:
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Images Ratings Applications Species Date Details
Western Blot CRHR1/CRF1 NBP2-16010
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reviewed by:
Anonymous
WB Other 09/11/2014
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Summary

ApplicationWestern Blot
Sample TestedBrain
SpeciesOther

Blocking

Blocking Details5% milk in TTBS

Primary Anitbody

Dilution Ratio1:500, 2nights, 4C, 5% milk in TTBS

Secondary Antibody

Secondary DescriptionIRDye680LT goat anti-rabbit
Secondary Manufacturer Cat#Li-Cor 926-68021
Secondary Concentration1:20K

Details

Detection NotesOdyssay Scanner

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CRHR1/CRF1 Antibody (NBP2-16010) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for CRHR1/CRF1 Antibody (NBP2-16010)

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Recent Reviews

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5
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4
1
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2
0
1
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Anonymous
09/11/2014
Application: WB
Species: Other

Bioinformatics

Gene Symbol CRHR1
Entrez
Uniprot