CRHR1/CRF1 Antibody

Western Blot: CRHR1/CRF1 Antibody [NBP2-16010] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: CRHR1/CRF1 Antibody [NBP2-16010] - HeLa xenograft, using CRFR1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Discontinued

CRHR1/CRF1 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CRHR1/CRF1. The exact sequence is proprietary.
• Localization: Cell membrane
• Predicted Species: Rat (100%), Rabbit (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CRHR1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID:33344730)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CRHR1/CRF1 Antibody

• CHR-R1
• corticotropin releasing hormone receptor 1
• corticotropin-releasing factor receptor 1
• corticotropin-releasing factor type 1 receptor
• Corticotropin-releasing hormone receptor 1
• CRF1
• CRFR
• CRF-R
• CRFR1
• CRF-R1
• CRF-R-1
• CRFR-1
• CRHR1
• CRH-R-1
• CRHR1f
• CRH-R1h
• CRHR1L
• CRHRCRH-R1
• seven transmembrane helix receptor

Background

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for CRHR1/CRF1 Antibody (NBP2-16010)(2)

Publications using NBP2-16010

• M D, Cs M, Gn P et al. Social isolation alters behavior, the gut-immune-brain axis, and neurochemical circuits in male and female prairie voles Neurobiology of Stress 2020-11-01 [PMID: 33344730] (WB, Mammal)
  
  Details:
  Prairie vole brain proteins were analyzed by Western blot analysis.
• Locci A, Yan Y, Rodriguez G, Dong H Sex-differences in CRF1, CRF, and CRFBP expression in C57BL/6J mouse brain across the lifespan and in response to acute stress J. Neurochem. 2020-08-19 [PMID: 32813270]

Review for CRHR1/CRF1 Antibody (NBP2-16010) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer WB Other 09/11/2014

Summary

• Application: Western Blot
• Sample Tested: Brain
• Species: Other

Bioinformatics

• Gene Symbol: CRHR1
• Uniprot:
  • P34998(Human)