CRABP2 Antibody (OTI10D6) - Azide and BSA Free

Western Blot: CRABP2 Antibody (OTI10D6) - Azide and BSA Free [NBP2-70448] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRABP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRABP2.

Immunohistochemistry: CRABP2 Antibody (OTI10D6) - Azide and BSA Free [NBP2-70448] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CRABP2 mouse monoclonal antibody.

Flow Cytometry: CRABP2 Antibody (OTI10D6) - Azide and BSA Free [NBP2-70448] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-CRABP2 antibody, and then analyzed by flow cytometry.

Western Blot: CRABP2 Antibody (OTI10D6) - Azide and BSA Free [NBP2-70448] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-CRABP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI10D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

CRABP2 Antibody (OTI10D6) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CRABP2 Antibody (OTI10D6) - Azide and BSA Free (NBP2-03807) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human CRABP2 (NP_001869) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRABP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
• Theoretical MW: 15.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for CRABP2 Antibody (OTI10D6) - Azide and BSA Free

• cellular retinoic acid binding protein 2
• Cellular retinoic acid-binding protein II
• CRABP2
• CRABP-II
• CRABP-IIcellular retinoic acid-binding protein 2
• RBP6

Background

Cellular retinoic acid binding protein 2 (CRABP2) is a coactivator present in the retinoic acid (RA)-dependent nuclear complex. CRABP2 has been shown to mediate retinoic acid activity in skin to influence skin cell growth and differentiation. It has also been shown to mediate the growth suppressive effects of retinoic acid in breast cancer. CRABP2's role in cell differentiation may be linked to control of the cell-cycle since it has been identified as an interacting partner of cyclin D3 in CRABPII and RAR ternary complex. Alternate names for CRABP2 include cellular retinoic acid-binding protein II, CRABP-II, retinoic acid-binding protein II, and RBP6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRABP2