CRABP2 Antibody (OTI10D6)

Western Blot: CRABP2 Antibody (10D6) [NBP2-03807] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-CRABP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry-Paraffin: CRABP2 Antibody (10D6) [NBP2-03807] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CRABP2 mouse monoclonal antibody.

Flow Cytometry: CRABP2 Antibody (10D6) [NBP2-03807] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CRABP2 antibody, and then analyzed by flow cytometry.

Western Blot: CRABP2 Antibody (10D6) [NBP2-03807] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRABP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRABP2.

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC
• Clone: OTI10D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CRABP2 Antibody (OTI10D6) Summary

• Immunogen: Full length human recombinant protein of human CRABP2 (NP_001869) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRABP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 15.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CRABP2 Antibody (OTI10D6)

• cellular retinoic acid binding protein 2
• Cellular retinoic acid-binding protein II
• CRABP2
• CRABP-II
• CRABP-IIcellular retinoic acid-binding protein 2
• RBP6

Background

This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRABP2
• Entrez:
  • 1382(Human)