CRABP1 Antibody (C-1)

Images

 
Immunocytochemistry/ Immunofluorescence: CRABP1 Antibody (C-1) [NB300-539] - Analysis of CRABPI using Anti-CRABPI Monoclonal Antibody (C-1) shows staining in NIH-3T3 Cells. CRABPI staining (green), F-Actin staining with ...read more
Immunohistochemistry-Paraffin: CRABP1 Antibody (C-1) [NB300-539] - Both normal and cancer biopsies of deparaffinized Human thyroid tissues.
Flow Cytometry: CRABP1 Antibody (C-1) [NB300-539] - Analysis of CRABPI in Hela cells (green) compared to an isotype control (blue).
Immunocytochemistry/ Immunofluorescence: CRABP1 Antibody (C-1) [NB300-539] - Analysis of CRABPI using Anti-CRABPI Monoclonal Antibody (C-1) shows staining in Hela Cells. CRABPI staining (green), F-Actin staining with ...read more
Immunocytochemistry/ Immunofluorescence: CRABP1 Antibody (C-1) [NB300-539] - Analysis of CRABPI using Anti-CRABPI Monoclonal Antibody (C-1) shows staining in MCF-7 Cells. CRABPI staining (green), F-Actin staining with ...read more
Immunohistochemistry-Paraffin: CRABP1 Antibody (C-1) [NB300-539] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.
Flow Cytometry: CRABP1 Antibody (C-1) [NB300-539] - Analysis of CRABPI in NIH-3T3 cells (green) compared to an isotype control (blue).
Flow Cytometry: CRABP1 Antibody (C-1) [NB300-539] - Analysis of CRABPI in MCF-7 cells (green) compared to an isotype control (blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, Fe, Pm, RbSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
C-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.

Order Details

CRABP1 Antibody (C-1) Summary

Immunogen
Oxidized CRABPI from bovine retina.
Specificity
Detects cellular Retinoic Acid Binding Protein 1 (CRABP 1). This does not cross-react with Cellular Retinol Binding Protein (CRBP), performic acid oxidized CRBP, interphotoreceptor retinoid binding protein or retinol binding protein.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CRABP1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1 - 2 ug
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:200
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:250
  • Immunohistochemistry-Paraffin 1:1000
Application Notes
WB: Detects an approx. 16 kDa protein representing CRABP 1 from rat retinal supernatant. IHC: Staining of CRABP 1 in rat retina results in its localization to amacrine somata and laminae in the inner plexiform layer.
Publications
Read Publications using NB300-539.

Reactivity Notes

Cross-reactivity with Reptiles.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites diluted with PBS
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Purity
Unpurified

Alternate Names for CRABP1 Antibody (C-1)

  • cellular retinoic acid binding protein 1
  • CRABP
  • CRABPI
  • CRABP-Icellular retinoic acid-binding protein 1
  • RBP5Cellular retinoic acid-binding protein I

Background

Retinoic acid (RA), a metabolite of vitamin A, plays an important role in growth and differentiation by modulation of expression of certain genes. RA functions through its interaction with the nuclear protein, retinoic acid receptor (RAR) and the 9-cis RA with retinoid X receptor (RXR). The Cellular Retinoic Acid Binding Proteins (CRABP) bind RA with a high affinity and belong to the family of fatty acid binding proteins. Two forms of CRABP have been characterized, CRABP 1 and CRABP 2 which share ~75% sequence conservation. The main role of CRABP 1 may be to protect retinoids from non-specific dehydrogenases and direct them to specific enzymes. The precise role of CRABP 2 is still poorly understood but it seems to play a role in the interaction of ligands (RA, 9-cis RA) with nuclear receptors (RAR/RXR).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CRABP1 Antibody (NB300-539) (0)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CRABP1
Entrez