COX-2 Antibody (495222) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
495222
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

COX-2 Antibody (495222) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human COX-2
Ala18-Ser112 and Gln386-Leu604
Accession # P35354
Specificity
Detects human COX-2 in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PTGS2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Control
COX-2 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
MAB4198 in the following applications:

Publications
Read Publications using
MAB4198 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for COX-2 Antibody (495222) [Unconjugated]

  • COX2
  • COX-2
  • COX2cyclooxygenase 2b
  • cyclooxygenase-2
  • EC 1.14.99
  • EC 1.14.99.1
  • GRIPGHS
  • hCox-2
  • PGG/HS
  • PGH synthase 2
  • PGHS-2
  • PHS II
  • PHS-2
  • PHS-II
  • prostaglandin G/H synthase 2
  • prostaglandin G/H synthase and cyclooxygenase
  • Prostaglandin H2 synthase 2
  • prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase andcyclooxygenase)
  • Prostaglandin-endoperoxide synthase 2
  • PTGS2

Background

Cyclooxygenase-2 (COX-2) also known as prostaglandin G/H synthase 2 (PGHS2) is a 70 kDa microsomal enzyme that belongs to the prostaglandin G/H synthase family. It is inducibly-expressed by a number of cell types, including fibroblasts, vascular smooth muscle cells, endothelium, and monocytes. Functionally, COX-2 is a homodimer that catalyzes two steps in the conversion of arachadonic acid to prostaglandin H2. Mature human COX-2 is 587 amino acids (aa) in length and contains one EGF-like domain (aa 18‑55), a potential membrane interacting region (aa 277‑292) and a globular catalytic domain (aa 293‑604). At least one splice form exists that shows an 11 aa substitution for the C-terminal 451 amino acids. Over the amino acid range of the immunogen, human COX-2 shows 83% aa identity to mouse COX-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for COX-2 Antibody (MAB4198)(2)

Review for COX-2 Antibody (MAB4198) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB4198:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin COX-2 MAB4198
Enlarge
4
reviewed by:
Anonymous
IHC-P Human 01/16/2020
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman Tonsil tissue
SpeciesHuman
LotCBLK0116091

Comments

CommentspH 9 antigen retrieval

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for COX-2 Antibody (MAB4198) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on COX-2.

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
01/16/2020
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol PTGS2
Uniprot