Connexin 40/GJA5 Overexpression Lysate

Images

 
Western Blot: GJA5 Overexpression Lysate (Adult Normal) [NBL1-11090] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GJA5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Connexin 40/GJA5 Overexpression Lysate Summary

Description

Connexin 40/GJA5 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC221330

Accession#: NM_181703

Protein Tag: C-MYC/DDK
Gene
GJA5

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
40.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Connexin 40/GJA5 Overexpression Lysate

  • alpha 5, 40kD (connexin 40)
  • ATFB11
  • Connexin 40
  • connexin-40
  • CX40
  • gap junction alpha-5 protein
  • gap junction protein, alpha 5, 40kDa (connexin 40)
  • gap junction protein, alpha 5, 40kDa
  • GJA5
  • MGC11185

Background

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described. Transcript Variant: This variant (B) differs in the 5' UTR compared to variant A. Variants A and B encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Connexin 40/GJA5 Lysate (NBL1-11090) (0)

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Bioinformatics

Gene Symbol GJA5