Human Connexin 37/GJA4 ELISA Kit (Colorimetric)

ELISA: Human Connexin 37/GJA4 ELISA Kit (Colorimetric) [NBP2-79829] - Samples were spiked with high concentrations of Human Connexin 37/GJA4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Connexin 37/GJA4 ELISA Kit (Colorimetric) [NBP2-79829] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: serum, plasma, and other biological fluids
• Standard Curve Range: 0.16-10 ng/mL
• Sensitivity: 0.10 ng/mL

Human Connexin 37/GJA4 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human CX37 in samples. No Significant cross-reactivity or interference between Human CX37 and analogues was observed.
• Standard Curve Range: 0.16-10 ng/mL
• Sensitivity: 0.10 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.79%
• Intra-Assay: CV% < 5.7%
• Spike Recovery: 88-110%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: GJA4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Connexin 37/GJA4 ELISA Kit (Colorimetric)

• Connexin 37
• Connexin-37
• CX37
• CX37connexin-37
• gap junction alpha-4 protein
• gap junction protein, alpha 4, 37kD (connexin 37)
• gap junction protein, alpha 4, 37kDa (connexin 37)
• gap junction protein, alpha 4, 37kDa
• GJA4

Background

FUNCTION: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. SUBUNIT: A connexon is composed of a hexamer of connexins. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein. TISSUE SPECIFICITY: Highly expressed in lung. SIMILARITY: Belongs to the connexin family. Alpha-type (group II) subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Connexin 37/GJA4 ELISA Kit (NBP2-79829). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: GJA4
• Uniprot:
  • P35212(Human)
  • P35212(Human)