Complement Component C9 Antibody (C9 53) - BSA Free

Product Discontinued

Complement Component C9 Antibody (C9 53) - BSA Free Summary

• Immunogen: Complement factor C9 adsorbed onto aluminum hydroxide gel
• Specificity: Complement component C9
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: C9
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Western Blot
• Application Notes: ELISA Recognizes C9 in human serum diluted 1:50 in Tris buffer (20 mM Tris-base, 1 mM MgCl2, 1 mM CaCl2 and 140 mM NaCl) and incubated for 2 hours at 37C using a human IgM coated (10 ug/mL overnight at 4C, blocked with PBS 7.2 + 1% BSA for 1 hour)

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Complement Component C9 Antibody (C9 53) - BSA Free

• complement component 9
• Complement Component C9

Background

Complement factor C9 is a 71-kDa single-chain ellipsoid a-globular molecule of 558 amino acids including a leader sequence of 20 amino acids. C9 bind the C5b8 complex on membranes and have an unique property in forming tubular structures containing between 12 and 18 monomers, closely resembling the membrane attack complex (MAC). Primary site of synthesis is hepatocytes, secondary sites are monocytes, fibroblasts and glial cells. The normal level of C9 in plasma is 60 ug/mL, and up regulated from hepatocytes by IFN-g.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C9