Complement Component C3aR Antibody (3G7) - Chimeric - Azide and BSA Free

Immunocytochemistry /Immunofluorescence: Complement Component C3aR Antibody (3G7) - Chimeric - Azide and BSA Free [NBP2-52674] - Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton and stained with NBP2-52674 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52674, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.

• Reactivity: Hu
• Applications: ELISA, Flow, ICC/IF, CyTOF-ready
• Clone: 3G7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Complement Component C3aR Antibody (3G7) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: The 2e loop of the C3a receptor.
• Specificity: Binds specifically to the 2e loop of the human C3a receptor, residues 185-193.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: C3AR1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: This antibody is Cytof ready. This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques. This scFv antibody binds to the 2e loop of the human Complement Component C3a recceptor, a G protein-coupled receptor stimulating chemotaxis, superoxide anion generation and granule enzyme release.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Complement Component C3aR Antibody (3G7) - Chimeric - Azide and BSA Free

• AZ3B
• AZ3BC3a anaphylatoxin chemotactic receptor
• C3AR
• C3a-R
• C3AR1
• C3ARcomplement component 3 receptor 1
• C3R1
• complement component 3a receptor 1
• Complement Component C3a R
• Complement Component C3aR
• HNFAG09

Background

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis,granule enzyme release and superoxide anion production

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C3AR1