F0101B). View our protocol for Staining Membrane-associated Proteins. " alt="HEK293 human embryonic kidney cell line transfected with human C3aR and eGFP (A) vs irrelevant transfectant (B) were stained with Mouse Anti-Human Complement Component C3aR Monoclonal Antibody (Catalog # MAB5995) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52674-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64835-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81062-0.025mg | |||
![Immunohistochemistry-Paraffin: Complement Component C3aR Antibody [NBP2-15649] - Complement Component C3a R Antibody [NBP2-15649] - Tissue staining sections of mouse lung tissue without (left) and with (right) silencing by siRNA .Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Complement-Component-C3a-R-Antibody-Immunohistochemistry-Paraffin-NBP2-15649-img0007.jpg "<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Complement Component C3aR Antibody [NBP2-15649] - Complement Component C3a R Antibody [NBP2-15649] - Tissue staining sections of mouse lung tissue without (left) and with (right) silencing by siRNA .Image submitted by a verified customer review.")
![<b>Genetic Strategies Validation. </b>Western Blot: Complement Component C3aR Antibody [NBP2-15649] - Boiled HL-60 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Complement Component C3aR antibody (NBP2-15649) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15649_rabbit-complement-component-c3ar-pab-910202420131262.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Complement Component C3aR Antibody [NBP2-15649] - Non-transfected (–) and transfected (+) unboiled HL-60 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C3a Receptor antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15649_rabbit-complement-component-c3ar-pab-1610202419405768.jpg)
MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # ![Flow Cytometry: Complement Component C3aR Antibody (HC3aRZ8) [NBP3-14618] - Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human C3aR (HC3aRZ8) purified antibody (concentration in sample 1,7 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/Complement-Component-C3aR-Antibody-HC3aRZ8-Flow-Cytometry-NBP3-14618-img0002.jpg "Flow Cytometry: Complement Component C3aR Antibody (HC3aRZ8) [NBP3-14618] - Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human C3aR (HC3aRZ8) purified antibody (concentration in sample 1,7 ug/ml, GAM APC).")
![Flow Cytometry: Complement Component C3aR Antibody (HC3aRZ8) [NBP3-14618] - Separation of human neutrophil granulocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human C3aR (HC3aRZ8) purified antibody (concentration in sample 1,7 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/Complement-Component-C3aR-Antibody-HC3aRZ8-Flow-Cytometry-NBP3-14618-img0001.jpg)
![Western Blot: C3a receptor 1 Overexpression Lysate (Adult Normal) [NBP2-04261] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for C3a receptor 1.](http://images.novusbio.com/fullsize/C3a-receptor-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04261-img0001.jpg "Western Blot: C3a receptor 1 Overexpression Lysate (Adult Normal) [NBP2-04261] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for C3a receptor 1.")
![Immunocytochemistry /Immunofluorescence: Complement Component C3aR Antibody (3G7) - Chimeric - Azide and BSA Free [NBP2-52674] - Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton and stained with NBP2-52674 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52674, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-52674_rabbit-monoclonal-complement-component-c3ar-antibody-3g7-chimeric-74202522113043.png "Immunocytochemistry /Immunofluorescence: Complement Component C3aR Antibody (3G7) - Chimeric - Azide and BSA Free [NBP2-52674] - Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton and stained with NBP2-52674 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-52674, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
