Complement Component C1r Antibody (269104)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 269104
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Complement Component C1r Antibody (269104) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Complement Component C1r
  Ser18-Asp705
  Accession # NP_001724
• Specificity: Detects human C1r in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) C1s or rhC1q R1 is observed. In Western blots, ths antibody recognizes rhC1r and the A/heavy chain.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: C1R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Component C1r Antibody (269104)

• C1r
• complement C1r subcomponent
• Complement component 1 subcomponent r
• complement component 1, r subcomponent
• Complement Component C1r
• EC 3.4.21
• EC 3.4.21.41

Background

The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. Following the C1q recognition, C1r is autoactivated, and in turn activates C1s, which cleaves C4 and C2, the C1 substrates (1). Both C1r and C1s activation involve cleavage of a specific Arg-Ile bond, converting single-chain proenzymes into active proteases of disulfide bond-linked chains (A and B) (2). The A chains contain multiple domains in the order of CUB1-EGF-CUB2-CCP1-CCP2-Activation Peptide. The B chains contain the serine protease catalytic domain.

1. Arlaud, G.J. et al. (2002) Biochem. Soc. Trans. 30:1001.
2. Lacroix, M. et al. (2001) J. Biol. Chem. 276:36233.
3. Journet A. and M. Tosi (1986) Biochem. J. 240:783.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: C1R
• Uniprot:
  • NP_001724()