Collagen XXV alpha 1 Antibody (540802) [Unconjugated]

• Reactivity: Hu
• Applications: IHC
• Clone: 540802
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Collagen XXV alpha 1 Antibody (540802) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Collagen XXV alpha 1
  Gly189-Lys654
  Accession # NP_942014
• Specificity: Detects human Collagen XXV alpha 1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) COL1A1, rhCOL3A1, rhCOL4A1, rhCOL13A1, rhCOL23A1 and recombinant mouse COL13A1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: COL25A1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Collagen XXV alpha 1 Antibody (540802) [Unconjugated]

• Alzheimer disease amyloid-associated protein
• AMY
• CLAC
• CLACP
• CLAC-P
• COL25A1
• Collagen 25
• collagen alpha-1(XXV) chain
• Collagen XXV alpha 1
• collagen, type XXV, alpha 1
• Collagen-25
• collagen-like Alzheimer amyloid plaque component
• collagenous Alzheimer amyloid plaque component

Background

Collagen XXV (Collagen 25 alpha 1 chain; also CLAC-P) is a neuronal, 65‑75 kDa member of the Collagenous transmembrane (TM) subfamily of the Collagen protein family. It is synthesized as a 654 amino acid (aa) type II TM glycoprotein that contains a 33 aa cytoplasmic region (aa 1‑33) and a 600 aa extracellular domain (ECD) (aa 55‑654). The ECD contains seven Collagen-like domains. Furin cleavage between Arg112‑Glu113 generates a 70 kDa soluble fragment that homotrimerizes and binds to early A beta amyloid deposits. There are at least five multiple splice forms that impact the ECD. Amino acids 139‑146 and the seventh Collagen-like domain (aa 571‑630) are most often affected. Over aa 189‑654, human COL25A1 is 95% aa identical to mouse COL25A1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COL25A1
• Uniprot:
  • NP_942014()