Cofilin Antibody

Images

 
Western Blot: Cofilin Antibody [NB100-81866] - Analysis of extracts from MDA cells using cofilin (Ab-3)
Immunohistochemistry: Cofilin Antibody [NB100-81866] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin (Ab- 1022) antibody.
Western Blot: Cofilin Antibody [NB100-81866] - Analysis of extracts from COLO 205 cells using cofilin (Ab-1022) antibody (Lane 1 and 2) and cofilin (phospho-Ser3) antibody (Lane 3 and 4).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Cofilin Antibody Summary

Immunogen
Peptide sequence around aa. 1~5 (M-A-S-G-V) derived from Human cofilin.
Specificity
cofilin (specific to a.a. 1022) detects endogenous levels of total cofilin protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CFL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Cofilin Lysate (NBL1-09126)
Publications
Read Publication using NB100-81866.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cofilin Antibody

  • 18 kDa phosphoprotein
  • CFL
  • cofilin 1 (non-muscle)
  • Cofilin, non-muscle isoform
  • cofilin-1
  • p18

Background

Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their 'pointed' (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH-dependent, Ca2+- independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cofilin Antibody (NB100-81866)(1)

We have publications tested in 1 confirmed species: Mouse.


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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Cofilin.

ACTB - an abundant cytoskeletal component with applications for gene expression analysis
Actin is the widely studied and ubiquitous cytoskeletal protein capable of forming dynamic microfilament structures. These filaments are essential for diverse cellular functions including cell shape, migration, cytokinesis, and intracellular traffi...  Read full blog post.

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Bioinformatics

Gene Symbol CFL1
Entrez
Uniprot