Coatomer Subunit Delta Antibody

Images

 
Western Blot: Coatomer Subunit Delta Antibody [NBP1-32377] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COPD antibody.
Immunocytochemistry/ Immunofluorescence: Coatomer Subunit Delta Antibody [NBP1-32377] - Paraformaldehyde-fixed HeLa, using COPD(NBP1-32377) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with ...read more
Western Blot: Coatomer Subunit Delta Antibody [NBP1-32377] - Sample(30 ug whole cell lysate)A:H1299B:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:500.
Western Blot: Coatomer Subunit Delta Antibody [NBP1-32377] - analysis of ARCN1 in CRISPR knock-downs Day 7 (3 different target sequences) and KMS11 wt using anti-ARCN1 antibody. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, RMSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.21 mg/ml
     

Genetic Strategies

   

Order Details

Coatomer Subunit Delta Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human COPD. The exact sequence is proprietary.
Localization
Cytoplasm, Golgi apparatus membrane, Peripheral membrane protein, Cytoplasmic side, Cytoplasmic vesicle, COPI-coated vesicle membrane, Peripheral membrane protein, Cytoplasmic side, Cytoplasm, Golgi apparatus membrane, Cytoplasmic vesicle, COPI-coa
Predicted Species
Mouse (98%), Rat (98%), Chicken (92%), Bovine (97%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARCN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-32377 in the following applications:

Publications
Read Publications using
NBP1-32377 in the following applications:

  • WB
    2 publications

Reactivity Notes

Xenopus laevis (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.21 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Coatomer Subunit Delta Antibody

  • archain 1
  • archain vesicle transport protein 1
  • Archain
  • coatomer delta subunit
  • coatomer protein complex, subunit delta
  • coatomer protein delta-COP
  • coatomer subunit delta
  • COPD
  • Delta-coat protein
  • delta-COP

Background

This gene maps in a region, which include the mixed lineage leukemia and Friend leukemia virus integration 1 genes, where multiple disease-associated chromosome translocations occur. It is an intracellular protein. Archain sequences are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Coatomer Subunit Delta Antibody (NBP1-32377)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Filter By Species
Human
(2)
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Review for Coatomer Subunit Delta Antibody (NBP1-32377) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-32377:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Coatomer Subunit Delta NBP1-32377
Enlarge
reviewed by:
Natalie Erdmann
WB Human 08/24/2015
View

Summary

ApplicationWestern Blot
Sample TestedKMS11 wt, CRISPR knock-downs Day 7 (3 different target sequences)
SpeciesHuman
Lot39918

Blocking

Blocking Details1% BSA in TBS-T

Primary Anitbody

Dilution Ratio1/1000 primary antibody diluted in TBS-T + 1% BSA; incubated overnight at 4oC

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#Cell Signal 7074S
Secondary Concentration1/10,000

Details

Detection NotesECL detection (GE Healthcare#RPN2106), exposure 1.5 minutes on Amersham ECL Hyperfilm.

Comments

CommentsLysis buffer: 150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 50mM Hepes pH7.5

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Coatomer Subunit Delta Antibody (NBP1-32377) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

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Bioinformatics Tool for Coatomer Subunit Delta Antibody (NBP1-32377)

Discover related pathways, diseases and genes to Coatomer Subunit Delta Antibody (NBP1-32377). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Coatomer Subunit Delta Antibody (NBP1-32377)

Discover more about diseases related to Coatomer Subunit Delta Antibody (NBP1-32377).
 

Pathways for Coatomer Subunit Delta Antibody (NBP1-32377)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Natalie Erdmann
08/24/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol ARCN1
Entrez
Uniprot