COPB2 Antibody (M3A5)

• Reactivity: Hu, Rt, Av, Bv, Ca, Ch, Ha, Pm, Pm, Rb
• Applications: WB, ICC/IF, IP, AP
• Clone: M3A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

COPB2 Antibody (M3A5) Summary

• Immunogen: Microtubule-associated protein from goose brain.
• Localization: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
• Specificity: Recognizes an epitope shared by the beta-COP protein (110kD) found in most tissue culture cells, and a high MW doublet of brain microtubule-associated protein (MAP 2, 270-300 kD).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: COPB2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunoaffinity Purification
  • Immunocytochemistry/Immunofluorescence 1:20-1:50
  • Immunoprecipitation 1:10-1:500
  • Western Blot
• Application Notes: AF: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: CHO cells IP: Use at an assay dependant dilution. Stacked Golgi membranes from rat liver extract. Stains a reticular structure in the perinuclear area of non-neuronal cells (the periphery of the Golgi complex and a population of vesicles scattered throughout the cytoplasm.

Reactivity Notes

kangaroo rat, goose

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 15mM Sodium Azide
• Purity: Unpurified

Alternate Names for COPB2 Antibody (M3A5)

• beta prime subunit
• Beta'-coat protein
• Beta'-COP
• betaprime-COP
• coatomer protein complex, subunit beta 2 (beta prime)
• coatomer subunit beta'
• p102

Background

May be used for studies on the effects of various agents influencing energy status, on disrupting the Golgi complex, or on altering the activity of G-proteins or small GTP-binding proteins on the cellular localization of beta-COP, and for the immunoaffinity purification of the beta-COP protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for COPB2 Antibody (NB600-102)(5)

Publications using NB600-102

• Cianciola NL, Carlin CR. Adenovirus RID-alpha activates an autonomous cholesterol regulatory mechanism that rescues defects linked to Niemann-Pick disease type C. J Cell Biol187(4):537-52. 2009-11-16 [PMID: 19948501]
• McGugan, G et al. Characterization of a Rab11 -like GTPase, EhRab11, of Entamoeba histolytica. Molecular Biochemical Parasitology 1: 1-10. 2003-01-01 [PMID: 12850258]
• Welter, B et al. Characterization of a Rab7-like GTPase, EhRab7: a marker for the earky stages of endocytosis in Entamoeba histolytica. Molecular Biochemical Parasitology 121:254-264. 2002-01-01 [PMID: 12034459]
• Donaldson JG, Lippincott-Schwartz J, Klausner RD. Guanine nucleotides modulate the effects of brefeldin A in semipermeable cells: regulation of the association of a 110-kD peripheral membrane protein with the Golgi apparatus. J Cell Biol112(4):579-88. 1991-02-01 [PMID: 1993732] (ICC/IF, Bovine, Rat)
• Donaldson JG, Lippincott-Schwartz J, Bloom GS et al. Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action. J Cell Biol111(6 Pt 1):2295-306. 1990-12-01 [PMID: 227706] (ICC/IF, Rat)

Bioinformatics

• Gene Symbol: COPB2
• Entrez:
  • 9276(Human)
  • 60384(Rat)