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COPB2 Antibody (M3A5)

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Product Details

Summary
Reactivity Hu, Rt, Av, Bv, Ca, Ch, Ha, Pm, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IP, AP
Clone
M3A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

COPB2 Antibody (M3A5) Summary

Immunogen
Microtubule-associated protein from goose brain.
Localization
The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
Specificity
Recognizes an epitope shared by the beta-COP protein (110kD) found in most tissue culture cells, and a high MW doublet of brain microtubule-associated protein (MAP 2, 270-300 kD).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
COPB2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoaffinity Purification
  • Immunocytochemistry/ Immunofluorescence 1:20-1:50
  • Immunoprecipitation 1:10-1:500
  • Western Blot
Application Notes
AF: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: CHO cells IP: Use at an assay dependant dilution. Stacked Golgi membranes from rat liver extract. Stains a reticular structure in the perinuclear area of non-neuronal cells (the periphery of the Golgi complex and a population of vesicles scattered throughout the cytoplasm.
Publications
Read Publications using
NB600-102 in the following applications:

Reactivity Notes

kangaroo rat, goose

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.9% Sodium Azide
Purity
Unpurified

Alternate Names for COPB2 Antibody (M3A5)

  • beta prime subunit
  • Beta'-coat protein
  • Beta'-COP
  • betaprime-COP
  • coatomer protein complex, subunit beta 2 (beta prime)
  • coatomer subunit beta'
  • p102

Background

May be used for studies on the effects of various agents influencing energy status, on disrupting the Golgi complex, or on altering the activity of G-proteins or small GTP-binding proteins on the cellular localization of beta-COP, and for the immunoaffinity purification of the beta-COP protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for COPB2 Antibody (NB600-102)(5)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for COPB2 Antibody (NB600-102) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol COPB2
Entrez