CLEC10A/CD301 Antibody (125A10.03) - Azide and BSA Free

Immunohistochemistry: CLEC10A/CD301 Antibody (125A10.03) [DDX0010P-100] - IHC staining of lungcryosectionwith 125A10.03

Flow Cytometry: CLEC10A/CD301 Antibody (125A10.03) [DDX0010P-100] - FACS analysisof monocyte-derivedDCs (GM+IL4) stained with125A10.03+ goat anti-mouse FITC

• Reactivity: Hu, Ca
• Applications: WB, Flow, Func, IHC, IP
• Clone: 125A10.03
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

CLEC10A/CD301 Antibody (125A10.03) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CLEC10A/CD301 Antibody (125A10.03) - Azide and BSA Free (DDX0010P-100) is a monoclonal antibody validated for use in IHC, WB, Flow and IP. Anti-CLEC10A/CD301 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: human ASGPR Ig fusion protein
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC10A
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface) 1:10-1:1000
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Fuctional Activity note: internalization of the receptor following its binding by the Ab and when incubated at 37 degrees C (as per Valladeau et al. 2010)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl (pH 8.0)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for CLEC10A/CD301 Antibody (125A10.03) - Azide and BSA Free

• CD301 antigen
• CD301
• CD301C-type lectin domain family 10 member A
• CLEC10A
• CLECSF13
• CLECSF14
• CLECSF14macrophage C-type lectin
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 14 (macrophage-derived)
• C-type lectin domain family 10, member A
• C-type lectin superfamily member 14
• HML2
• HMLC-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 13 (macrophage-derived)
• macrophage lectin 2 (calcium dependent)
• Macrophage lectin 2
• MGL

Background

CD301 encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell surface antigen. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLEC10A/CD301 Antibody (DDX0010P-100)(5)

Publications using DDX0010P-100

• Lei V, Handfield C, Kwock JT et al. Skin Injury Activates A Rapid TRPV1-dependent Antiviral Protein Response The Journal of investigative dermatology 2022-01-07 [PMID: 35007556]
• Garcia-Vallejo JJ et al. Langerin-mediated internalization of a modified peptide routes antigens to early endosomes and enhances cross-presentation by human Langerhans cells. Cell Mol Immunol 2015-10-01 [PMID: 26456691]
• Debeer S et al. Comparative histology and immunohistochemistry of porcine versus human skin. Eur J Dermatol 2013-07-01 [PMID: 24047577]
• Napoletano C et al. Targeting of macrophage galactose-type C-type lectin (MGL) induces DC signaling and activation. Eur J Immunol 2012-04-01 [PMID: 22531918]
• Valladeau J et al. Immature human dendritic cells express asialoglycoprotein receptor isoforms for efficient receptor-mediated endocytosis. J Immunol 2001-11-01 [PMID: 11698450]

Bioinformatics

• Gene Symbol: CLEC10A