Mouse CLC ELISA Kit (Colorimetric)

ELISA: Mouse CLC ELISA Kit (Colorimetric) [NBP2-75321] - Samples were spiked with high concentrations of Mouse CLC and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse CLC ELISA Kit (Colorimetric) [NBP2-75321] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL

Mouse CLC ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse CLCF1 in samples. No significant cross-reactivity or interference between Mouse CLCF1 and analogues was observed.
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.35%
• Intra-Assay: CV% < 4.43%
• Spike Recovery: 86-111%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CLCF1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse CLC ELISA Kit (Colorimetric)

• B-cell stimulating factor 3
• B-cell stimulatory factor 3
• BSF-3
• BSF3BSF-3
• cardiotrophin-like cytokine factor 1
• CISS2
• CLC
• CLCF1
• CLCNNT-1
• CRLF1 associated cytokine-like factor 1
• neurotrophin-1/B-cell stimulating factor-3
• NNT-1
• NNT1B-cell-stimulating factor 3
• Novel neurotrophin-1
• NR6

Background

The CLCF1 gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1(CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for CLC ELISA Kit (NBP2-75321)(1)

Publication using NBP2-75321

• Wang K, Wang S, Chen Y et al. Single-cell transcriptomic analysis of somatosensory neurons uncovers temporal development of neuropathic pain Cell research 2021-03-10 [PMID: 33692491] (ELISA, Mouse)

FAQs for CLC ELISA Kit (NBP2-75321). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CLCF1