CKS1 Antibody (AA10.2)

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Product Details

Summary
Reactivity YSpecies Glossary
Applications WB, IHC, IP
Clone
AA10.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CKS1 Antibody (AA10.2) Summary

Immunogen
p13
Localization
Cytoplasmic and Nuclear.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CKS1B
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for CKS1 Antibody (AA10.2)

  • CDC28 protein kinase 1
  • CDC28 protein kinase 1B
  • CDC28 protein kinase regulatory subunit 1B
  • cell division control protein CKS1
  • CKS-1
  • CKS1CDC2-associated protein CKS1
  • ckshs1
  • cyclin-dependent kinases regulatory subunit 1
  • NB4 apoptosis/differentiation related protein
  • PNAS-143
  • PNAS-16
  • PNAS-18

Background

SUC1 is a CDK subunit from S. pombe that interacts with CDK1. SUC1 is used exclusively to capture CDK1 complexes from various cell lines and egg extracts. SUC1 belongs to the CKS family. It binds to the catalytic subunit of the cyclin dependent kinase (cdc2) and is essential for its biological function. SUC1 forms a homodimer (in presence of zinc).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-2744
Species: Y
Applications: WB, IHC, IP

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CKS1 Antibody (NB100-2744). (Showing 1 - 1 of 1 FAQ).

  1. Regarding NB100-2744, the specificity statement says that this antibody can be used exclusively to capture CDK1 complexes from various cell lines and egg extracts. Does "exclusively" mean to imply that the antibody does not cross-react with CKS2? Is there any data regarding potential cross-reactivity? Also, I am a little bit confused. Isn't this clone (AA10.2) supposed to be an antibody against SUC1? SUC1 interacts with CDK1 and is used exclusively to capture CDK1 complexes from various cell lines and egg extracts. Am I correct in interpreting that antibody NB100-2744 is able to capture CDK1 complexes from various cell lines and egg extracts by virtue of its binding with SUC1 which is complexed with CDK1?
    • Yes, you are correct. This antibody will not cross-react with CKS2, and was raised against suc1 (or p13). We sell it as a product to purify CDK1 complexes although it doesn't actually bind CDK1.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CKS1B
Entrez
Uniprot