CIRBP Antibody

Western Blot: CIRBP Antibody [NBP1-52067] - NBP1-52067 (0.01ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: CIRBP Antibody [NBP1-52067] - (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CIRBP Antibody Summary

• Description: Novus Biologicals Goat CIRBP Antibody (NBP1-52067) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-CIRBP Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RDSYDSYATHNE corresponding to C-Terminus according to NP_001271.1.
• Epitope: C-RDSYDSYATHNE
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CIRBP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 18 kDa band observed in mouse and rat testis lysates (calculated MW of 18.6 kDa band according to human NP_001271.1 and mouse NP_031731.1). IHC-P: Human testis shows nuclear staining preferentially in Type A spermatogonia.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CIRBP Antibody

• A18 hnRNP
• A18HNRNP
• CIRPcold-inducible RNA-binding protein
• cold inducible RNA binding protein
• cold inducible RNA-binding protein
• glycine-rich RNA binding protein
• Glycine-rich RNA-binding protein CIRP

Background

Seems to play an essential role in cold-induced suppression of cell proliferation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CIRBP Antibody (NBP1-52067)(1)

Publication using NBP1-52067

• Zeng Y, Kulkarni P, Inoue T, Getzenberg RH. Down-regulating cold shock protein genes impairs cancer cell survival and enhances chemosensitivity. J Cell Biochem 2009-05-01 [PMID: 19277990]

Bioinformatics

• Gene Symbol: CIRBP
• Entrez:
  • 1153(Human)
  • 12696(Mouse)
  • 81825(Rat)
• Uniprot:
  • NP_001271.1(Human)