Chromogranin B Antibody (Sulfo-1C-A2)

Product Discontinued

Chromogranin B Antibody (Sulfo-1C-A2) Summary

• Immunogen: This antibody was developed using a phage display library as described in Kehoe et al., 2006. The selection antigens were KAKISDP-DY(SO3)MTGYMDAC and KDKKYATEY(SO3)-EYLDYDFC.
• Specificity: Recognizes sulfated tyrosine residues on various proteins. Does not recognize phosphotyrosine or unmodified tyrosines.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHGB
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Reactivity Notes

All

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7.5), 0.15M NaCl and 0.18M Glycine
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Chromogranin B Antibody (Sulfo-1C-A2)

• CgB
• CHGB
• chromogranin B (secretogranin 1)
• Chromogranin B
• Chromogranin-B
• SCG1
• SCG1sgI
• secretogranin B
• Secretogranin I
• secretogranin-1
• SgI

Background

Sulfotyrosine is a common post-translational modification of secretory, plasma membrane, and lysosomal proteins. The sulfation reaction is catalyzed by specific sulfotransferase enzymes in the trans-Golgi-network. Tyrosine sulfation occurs in all multicellular organisms and up to 1% of the tyrosines in the total protein content of a cell can be sulfated. Sulfotyrosines mediates many receptor-ligand binding interactions, including the interaction of P-selectin glycoprotein ligand-1 with P-selectin, factor VIII binding to von Willebrand factor, and interactions between thrombin and hirudin. Sulfotyrosine is also involved in the entry of several parasites and viruses; for example, tyrosine sulfation of the HIV-1 co-receptor CCR5 is required for viral entry into host cells, and tyrosine sulfation of the Duffy antigen/receptor for chemokines is crucial for erythrocyte invasion by the malaria parasite Plasmodium vivax. This clone was derived from the antibody described in Kehoe et al. , 2006.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHGB