Chondroitin sulfate synthase 3 Antibody

Western Blot: Chondroitin sulfate synthase 3 Antibody [NBP1-59673] - OVCAR-3 cell lysate, concentration 0.2-1 ug/ml.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Chondroitin sulfate synthase 3 Antibody Summary

• Immunogen: Synthetic peptides corresponding to CHSY-2 The peptide sequence was selected from the N terminal of CHSY-2. Peptide sequence PPLQQRRRGREPEGATGLPGAPAAEGEPEEEDGGAAGQRRDGRPGSSHNG. The peptide sequence for this immunogen was taken from within the described region.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHSY3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1.0 ug/ml
• Application Notes: This is a rabbit polyclonal antibody against CHSY-2 and was validated on Western blot.
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 2% Sucrose
• Preservative: 0.09% Sodium Azide
• Purity: Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for Chondroitin sulfate synthase 3 Antibody

• Carbohydrate synthase 2
• Chondroitin glucuronyltransferase 3
• chondroitin sulfate synthase 3
• Chondroitin synthase 2
• chondroitin synthase-2
• CHSY2
• chSy-2
• CSS3N-acetylgalactosaminyltransferase 3
• EC 2.4.1.175
• EC 2.4.1.226
• Glucuronosyl-N-acetylgalactosaminyl-proteoglycan4-beta-N-acetylgalactosaminyltransferase II
• N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase 3

Background

CHSY-2 has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. CHSY-2 transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Specific activity of CHSY-2 is much reduced compared to CHSY1.CSS3 is a glycosyltransferase that has both glucuronyltransferase and N-acetylgalactosaminyltransferase activities (Yada et al., 2003 [PubMed 12907687]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHSY3
• Uniprot:
  • Q70JA7(Human)