CHL-1/L1CAM-2 Antibody

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CHL-1/L1CAM-2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CHL‑1/L1CAM‑2
  Ile25-Gln1096
  Accession # EAW63869
• Specificity: Detects human CHL‑1/L1CAM‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant mouse CHL-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CHL1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CHL-1/L1CAM-2 Antibody

• CALL
• CALLClose homolog of L1
• cell adhesion molecule with homology to L1CAM (close homolog of L1)
• cell adhesion molecule with homology to L1CAM (close homologue of L1)
• CHL1
• CHL-1
• FLJ44930
• L1CAM-2
• L1CAM2L1 cell adhesion molecule 2
• MGC132578
• neural cell adhesion molecule L1-like protein

Background

Close homolog of L1 (CHL-1), also known as cell adhesion L1-like (CALL) and L1 cell adhesion molecule 2 (L1-CAM2), belongs to the L1 subfamily of the Ig superfamily cell adhesion molecules, which also include L1, neurofascin and NgCAM-related cell adhesion molecule (NrCAM) (1‑3). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4‑5 fibronectin type III-like (FNIII) domains in their extracellular regions. They also shared a highly conserved cytoplasmic region of approximately 110 amino acid residues (aa) containing an ankyrin-binding site. CHL-1 is expressed as a highly glycosylated 185 kDa transmembrane protein by subpopulations of neurons and glia of the central and peripheral nervous system (4, 5). Ectodomain shedding via the metalloprotease-disintegrin ADAM8 releases 165 kDa and 125 kDa soluble CHL-1 fragments, which can diffuse away to function at distant sites (6). CHL-1 is not capable of homotypic interactions, but an extracellular binding partner of CHL-1 has not been identified (4). Human CHL1 has been mapped to chromosome 3p26 and is a candidate gene for 3p^- syndrome characterized by mental impairment (7). A missense CHL1 polymorphism associated with an increased risk of schizophrenia has been reported (8). The functional importance of CHL-1 in the nervous system is also evident in CHL-1 deficient mice, which display behavioral abnormalities and show misguided axons within the hippocampus and olfactory tract (9). Enhanced ectodomain-shedding of CHL-1 is also observed in Wobbler mice, the neurodegenerative mutant mice (6). In vitro, soluble or substrate-coated CHL-1 promotes neurite outgrowth and neuronal survival of both cerebellar and hippocampal neurons. Cell surface CHL-1 interacts with integrins in cis to potentiate integrin-dependent cell migration toward extracellular matrix proteins (10). For this enhanced cell motility, CHL-1 linkage to the actin cytoskeleton via interaction between ankyrin and the CHL-1 cytoplasmic region is required.

1. Moos, M. et al. (1988) Nature 334:701. 
2. Holm, J. et al. (1996) Eur. J. Neusci. 8:1613. 
3. Wei, M. et al. (1998) Hum. Genet. 103:355. 
4. Hillenbrand, R. et al. (1999) Eur. J. Neurosci. 11:813. 
5. Liu, Q. et al. (2000) J. Neurosci. 20:7682. 
6. Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
7. Angeloni, D. et al. (1999) Am. J. Med. Genet. 86:482.
8. Sakurai, K. et al. (2002) Mol. Psychiatry 7:412.
9. Montag-Sallaz, M. et al. (2002) Mol. Cell. Biol. 22(22):7967.
10. Buhusi, M. et al. (2003) J. Biol. Chem. 278(27):25024.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHL-1/L1CAM-2 Antibody (AF2126)(2)

Publications using AF2126

• KF Karstens, E Bellon, A Polonski, G Wolters-Ei, N Melling, M Reeh, JR Izbicki, M Tachezy Expression and serum levels of the neural cell adhesion molecule L1-like protein (CHL1) in gastrointestinal stroma tumors (GIST) and its prognostic power Oncotarget, 2020-03-31;11(13):1131-1140. 2020-03-31 [PMID: 32284790] (IHC, Western Blot, Human)
• Robin Wachowiak, Steffi Mayer, Anne Suttkus, Illya Martynov, Martin Lacher, Nathaniel Melling et al. CHL1 and NrCAM are primarily expressed in low grade pediatric neuroblastoma Open Med (Wars) 12/31/2019 [PMID: 31989042]

Bioinformatics

• Gene Symbol: CHL1
• Uniprot:
  • EAW63869()