Chitotriosidase/CHIT1 Antibody

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Chitotriosidase/CHIT1 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Chitotriosidase/CHIT1
  Ala22-Asn466
  Accession # Q13231
• Specificity: Detects human Chitotriosidase/CHIT1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CHIT1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Chitotriosidase/CHIT1 Antibody

• CHI3
• CHIT1
• CHITCHI3
• chitinase 1 (chitotriosidase)
• chitinase-1
• Chitotriosidase
• chitotriosidase-1
• EC 3.2.1.14
• FLJ00314
• MGC125322
• plasma methylumbelliferyl tetra-N-acetylchitotetraoside hydrolase

Background

Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages (1). It is distinct from another member, known as acidic mammalian chitinase (AMC) encoded by the CHIA gene and expressed mainly in the gastrointestinal tract and lung (2). Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pHs and can be processed into a C-terminally truncated 39 kDa form (2, 3). CHIT1 is the best biomarker in the monitoring of Gaucher disease among the three most commonly used markers that also include acid phosphatase and angiotensin-converting enzyme (ACE) (4). CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke (5).

1. Aguilera, B. et al. (2003) J. Biol. Chem. 278:40911.
2. Boot, R.G. et al. (2001) J. Biol. Chem. 276:6770.
3. Renkema, G.H. et al. (1997) Eur. J. Biochem. 244:279.
4. Vellodi, A. et al. (2005) J. Inherit. Metab. Dis. 28:585.
5. Sotgiu, S. et al. (2005) Eur. Neurol. 54:149.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Chitotriosidase/CHIT1 Antibody (AF3559)(3)

Publications using AF3559

• Chang-Min Lee, Chuan-Hua He, Jin Wook Park, Jae Hyun Lee, Suchitra Kamle, Bing Ma et al. Chitinase 1 regulates pulmonary fibrosis by modulating TGF-beta /SMAD7 pathway via TGFBRAP1 and FOXO3 Life Science Alliance 6/1/2019 [PMID: 31085559]
• SJ Van Dyken, HE Liang, RP Naikawadi, PG Woodruff, PJ Wolters, DJ Erle, RM Locksley Spontaneous Chitin Accumulation in Airways and Age-Related Fibrotic Lung Disease Cell, 2017-04-20;169(3):497-509.e13. 2017-04-20 [PMID: 28431248] (Western Blot, Human)
• Agapov E, Battaile JT, Tidwell R, Hachem R, Patterson GA, Pierce RA, Atkinson JJ, Holtzman MJ Macrophage chitinase 1 stratifies chronic obstructive lung disease. Am. J. Respir. Cell Mol. Biol., 2009-06-02;41(4):379-84. 2009-06-02 [PMID: 19491341] (IHC-P, Human)

Bioinformatics

• Gene Symbol: CHIT1
• Uniprot:
  • Q13231()