Chitinase 3-like 2 Antibody (2B5) - BSA Free

Western Blot: Chitinase 3-like 2 Antibody (2B5) [NBP2-60238] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Chitinase 3-like 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: HeLa cell lysate Lane 3.: HepG2 cell lysate Lane 4.: LnCap cell lysate

Flow Cytometry: Chitinase 3-like 2 Antibody (2B5) [NBP2-60238] - Analysis of CHI3L2 in HeLa cells. The cell was stained with at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clone: 2B5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Chitinase 3-like 2 Antibody (2B5) - BSA Free Summary

• Description: Novus Biologicals Mouse Chitinase 3-like 2 Antibody (2B5) - BSA Free (NBP2-60238) is a monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human Chitinase 3-like 2 (27-390aa) purified from E. coli
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHI3L2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 2-5 ug
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Chitinase 3-like 2 Antibody (2B5) - BSA Free

• CHI3L2
• Chitinase 3 like 2
• Chitinase 3-like 2
• chitinase-3-like protein 2
• Chondrocyte protein 39
• YKL39
• YKL-39YKL39

Background

CHI3L2 may bind glycan structure with high affinity, but not heparin. Has no chitotriosidase activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHI3L2