Chemokine-like factor Overexpression Lysate (Native)

Images

 
Western Blot: Chemokine-like factor Overexpression Lysate (Adult Normal) [NBL1-09219] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Chemokine-like factor.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Chemokine-like factor Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210588 and based on accession number NM_181640. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chemokine-like factor (CKLF), transcript variant 2, mRNA.
Gene
CKLF

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
10.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Chemokine-like factor

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Chemokine-like factor Overexpression Lysate (Native)

  • C32CKLF2
  • chemokine-like factor 1
  • chemokine-like factor 2
  • chemokine-like factor 3
  • chemokine-like factor 4
  • chemokine-like factor
  • CKLF1
  • CKLF3
  • CKLF4
  • HSPC224
  • transmembrane proteolipid
  • UCK-1

Background

The product of this gene is a cytokine. Cytokines are small proteins that have an essential role in the immune and inflammatory responses. This gene is one of several chemokine-like factor genes located in a cluster on chromosome 16. The protein encoded by this gene is a potent chemoattractant for neutrophils, monocytes and lymphocytes. It also can stimulate the proliferation of skeletal muscle cells. This protein may play important roles in inflammation and in the regeneration of skeletal muscle. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (b), also known as an isoform CKLF1, that is missing an internal segment compared to isoform a.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol CKLF