CHD4 Antibody (4H4) - Azide and BSA Free

Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of CHD4 expression in transfected 293T cell line by CHD4 monoclonal antibody (M01), clone 4H4.Lane 1: CHD4 transfected lysate(220 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of monoclonal antibody to CHD4 on formalin-fixed paraffin-embedded human transitional cell carcinoma. Antibody concentration 3 ug/ml.

Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - CHD4 monoclonal antibody (M01), clone 4H4 Analysis of CHD4 expression in Hela S3 NE.

Sandwich ELISA: CHD4 Antibody (4H4) [H00001108-M01] - Detection limit for recombinant GST tagged CHD4 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 4H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CHD4 Antibody (4H4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CHD4 (NP_001264, 1632 a.a. ~ 1730 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWH
• Localization: Nuclear
• Specificity: CHD4 - chromodomain helicase DNA binding protein 4
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHD4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CHD4 Antibody (4H4) - Azide and BSA Free

• ATP-dependent helicase CHD4
• CHD-4
• chromodomain helicase DNA binding protein 4
• chromodomain-helicase-DNA-binding protein 4
• DKFZp686E06161
• EC 3.6.1
• EC 3.6.4.12
• Mi-2 autoantigen 218 kDa protein
• Mi-2b
• Mi2-BETA

Background

The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHD4 Antibody (H00001108-M01)(1)

Publication using H00001108-M01

• Nishibuchi G, Shibata Y, Hayakawa T et al. Physical and functional interactions between the histone H3K4 demethylase KDM5A and the nucleosome remodeling and deacetylase (NuRD) complex. J Biol Chem 2014-09-04 [PMID: 25190814]

Bioinformatics

• Gene Symbol: CHD4
• Entrez:
  • 1108(Human)
• OMIM:
  • 603277(Human)
• Uniprot:
  • NP_001264(Human)