![Western Blot: CHCHD2 Antibody [NBP2-92335] - Western blot analysis of extracts from normal (control) and CHCHD2 knockout (KO) 293T cells, using CHCHD2 antibody (NBP2-92335) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize2/CHCHD2-Antibody-Western-Blot-NBP2-92335-img0009.jpg "Western Blot: CHCHD2 Antibody [NBP2-92335] - Western blot analysis of extracts from normal (control) and CHCHD2 knockout (KO) 293T cells, using CHCHD2 antibody (NBP2-92335) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-P, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 75-145 of human CHCHD2 (NP_057223.1). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CHCHD2 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 50% glycerol, pH7.3. |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Western Blot: Huntingtin Antibody [NBP3-13106] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (NBP3-13106) diluted at 1:1000.](http://images.novusbio.com/images2/Huntingtin-Antibody-Western-Blot-NBP3-13106-img0001.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody [NBP3-13106] - Huntingtin antibody detects Huntingtin protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Huntingtin antibody (NBP3-13106) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/images2/Huntingtin-Antibody-Immunohistochemistry-Paraffin-NBP3-13106-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Huntingtin Antibody [NBP3-13106] - Huntingtin antibody detects Huntingtin protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Huntingtin protein stained by Huntingtin antibody (NBP3-13106) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/images2/Huntingtin-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13106-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: TRIM4 Antibody [NBP1-80824] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/images2/TRIM4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80824-img0009.jpg)
![Immunohistochemistry-Paraffin: TRIM4 Antibody [NBP1-80824] - Staining of human bone marrow shows distinct cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/images2/TRIM4-Antibody-Immunohistochemistry-Paraffin-NBP1-80824-img0004.jpg)
![Immunohistochemistry-Paraffin: TMEM111 Antibody [NBP1-86216] - Staining of human nasopharynx shows strong cytoplasmic and membranous positivity in respiratory epithelial cells.](http://images.novusbio.com/images2/TMEM111-Antibody-Immunohistochemistry-Paraffin-NBP1-86216-img0002.jpg)
![Flow (Intracellular): CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/images2/CD44-Antibody-(8E2F3)-Flow-(Intracellular)-NBP1-47386-img0016.jpg)
![Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/images2/CD44-Antibody-(8E2F3)-Flow-Cytometry-NBP1-47386-img0017.jpg)
![Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left).](http://images.novusbio.com/images2/CD44-Antibody-(8E2F3)-Flow-Cytometry-NBP1-47386-img0008.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for CHCHD2 Antibody (NBP2-92335)Discover more about diseases related to CHCHD2 Antibody (NBP2-92335).
| Pathways for CHCHD2 Antibody (NBP2-92335)View related products by pathway.
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PTMs for CHCHD2 Antibody (NBP2-92335)Learn more about PTMs related to CHCHD2 Antibody (NBP2-92335).
| Research Areas for CHCHD2 Antibody (NBP2-92335)Find related products by research area.
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| Gene Symbol | CHCHD2 |
![Immunocytochemistry/Immunofluorescence: CHCHD2 Antibody [NBP2-92335] - Analysis of C6 cells using CHCHD2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize2/CHCHD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92335-img0007.jpg "Immunocytochemistry/Immunofluorescence: CHCHD2 Antibody [NBP2-92335] - Analysis of C6 cells using CHCHD2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: CHCHD2 Antibody [NBP2-92335] - Immunohistochemistry of paraffin-embedded Rat ovary using [KO Validated] CHCHD2 Rabbit pAb (NBP2-92335) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize2/CHCHD2-Antibody-Immunohistochemistry-Paraffin-NBP2-92335-img0008.jpg "Immunohistochemistry-Paraffin: CHCHD2 Antibody [NBP2-92335] - Immunohistochemistry of paraffin-embedded Rat ovary using [KO Validated] CHCHD2 Rabbit pAb (NBP2-92335) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.")
![Western Blot: CHCHD2 Antibody [NBP2-92335] - Analysis of extracts of various cell lines, using CHCHD2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize2/CHCHD2-Antibody-Western-Blot-NBP2-92335-img0003.jpg "Western Blot: CHCHD2 Antibody [NBP2-92335] - Analysis of extracts of various cell lines, using CHCHD2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: CHCHD2 Antibody [NBP2-92335] - Analysis of L929 cells using CHCHD2 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize2/CHCHD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92335-img0001.jpg "Immunocytochemistry/Immunofluorescence: CHCHD2 Antibody [NBP2-92335] - Analysis of L929 cells using CHCHD2 at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: CHCHD2 Antibody [NBP2-92335] - Analysis of U-2 OS cells using CHCHD2 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize2/CHCHD2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92335-img0002.jpg "Immunocytochemistry/Immunofluorescence: CHCHD2 Antibody [NBP2-92335] - Analysis of U-2 OS cells using CHCHD2 at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: CHCHD2 Antibody [NBP2-92335] - Mouse kidney using [KO Validated] CHCHD2 Rabbit pAb (A16645) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize2/CHCHD2-Antibody-Immunohistochemistry-Paraffin-NBP2-92335-img0004.jpg "Immunohistochemistry-Paraffin: CHCHD2 Antibody [NBP2-92335] - Mouse kidney using [KO Validated] CHCHD2 Rabbit pAb (A16645) at dilution of 1:100 (40x lens).")
![Immunohistochemistry-Paraffin: CHCHD2 Antibody [NBP2-92335] - Human colon carcinoma using [KO Validated] CHCHD2 RabbitpAb (A16645) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize2/CHCHD2-Antibody-Immunohistochemistry-Paraffin-NBP2-92335-img0006.jpg "Immunohistochemistry-Paraffin: CHCHD2 Antibody [NBP2-92335] - Human colon carcinoma using [KO Validated] CHCHD2 RabbitpAb (A16645) at dilution of 1:100 (40x lens).")
![Western Blot: HABP1/C1QBP/GC1q R Antibody [NBP1-89790] - Analysis in human cell lines HEK293 and U2OS using Anti-C1QBP antibody. Corresponding C1QBP RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/images2/HABP1-C1QBP-GC1q-R-Antibody-Western-Blot-NBP1-89790-img0017.jpg)
![Immunohistochemistry-Paraffin: HABP1/C1QBP/GC1q R Antibody [NBP1-89790] - Staining of human colon shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/images2/HABP1-C1QBP-GC1q-R-Antibody-Immunohistochemistry-Paraffin-NBP1-89790-img0018.jpg)
![Immunohistochemistry-Paraffin: HABP1/C1QBP/GC1q R Antibody [NBP1-89790] - Staining of human lymph node shows strong granular cytoplasmic positivity in non-germinal and germinal center cells.](http://images.novusbio.com/images2/HABP1-C1QBP-GC1q-R-Antibody-Immunohistochemistry-Paraffin-NBP1-89790-img0019.jpg)
![Flow Cytometry: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [H00006532-D01P] - Fixed and permeabilized mouse mast cells were stained for 1 hour in 4C then stained with PE secondary and ran on FACS. Image from verified customer review.](http://images.novusbio.com/images2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-Flow-Cytometry-H00006532-D01P-img0006.jpg)
![Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [H00006532-D01P] - Analysis of SLC6A4 expression in transfected 293T cell line by SLC6A4 polyclonal antibody. Lane 1: SLC6A4 transfected lysate (70.30 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-Western-Blot-H00006532-D01P-img0004.jpg)
![Western Blot: SLC6A4/5-HTTLPR/Serotonin transporter Antibody [H00006532-D01P] - Analysis of SLC6A4 expression in mouse lung.](http://images.novusbio.com/images2/SLC6A4-5-HTTLPR-Serotonin-transporter-Antibody-Western-Blot-H00006532-D01P-img0003.jpg)
![Western Blot: PKI-beta Antibody [NBP1-74255] - Rat Kidney Lysate 1ug/ml Gel Concentration 10-20%](http://images.novusbio.com/images/PKI-beta-Antibody-Western-Blot-NBP1-74255-img0001.jpg)
![Immunohistochemistry-Paraffin: MDA5 Antibody [NB110-1244] - Staining in paraffin embedded Human Pancreas. Antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.](http://images.novusbio.com/images/MDA5-Antibody-Immunohistochemistry-Paraffin-NB110-1244-img0001.jpg)
![Flow Cytometry: MDA5 Antibody [NB110-1244] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/images2/MDA5-Antibody-Flow-Cytometry-NB110-1244-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SLIRP Antibody [NB110-37258] - SLIRP antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/images2/SLIRP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-37258-img0006.jpg)
![Immunohistochemistry: SLIRP Antibody [NB110-37258] - Analysis of SLIRP in mouse intestine using DAB with hematoxylin counterstain.](http://images.novusbio.com/images2/SLIRP-Antibody-Immunohistochemistry-NB110-37258-img0007.jpg)
![Simple Western: SLIRP Antibody [NB110-37258] - Simple Western lane view shows a specific band for SLIRP in 0.2 mg/ml of Human Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/images2/SLIRP-Antibody-Simple-Western-NB110-37258-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/images2/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0008.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.](http://images.novusbio.com/images2/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/images2/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0022.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")