Human CFTR ELISA Kit (Colorimetric)

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ELISA: Human CFTR ELISA Kit (Colorimetric) [NBP2-75258] - Samples were spiked with high concentrations of Human CFTR and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human CFTR ELISA Kit (Colorimetric) [NBP2-75258] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL

Order Details

Human CFTR ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human CFTR in samples. No significant cross-reactivity or interference between Human CFTR and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.10 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.79%
Intra-Assay
CV% < 5.5%
Spike Recovery
85-108%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CFTR

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human CFTR ELISA Kit (Colorimetric)

  • ABC35
  • ABCC7CF
  • ATP-binding cassette sub-family C member 7
  • ATP-binding cassette transporter sub-family C member 7
  • cAMP-dependent chloride channel
  • CFTR
  • CFTR/MRP
  • Channel conductance-controlling ATPase
  • cystic fibrosis transmembrane conductance regulator (ATP-binding cassettesub-family C, member 7)
  • cystic fibrosis transmembrane conductance regulator
  • cystic fibrosis transmembrane conductance regulator, ATP-binding cassette(sub-family C, member 7)
  • dJ760C5.1
  • EC 3.6.3
  • MRP7EC 3.6.3.49
  • TNR-CFTR

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for CFTR ELISA Kit (NBP2-75258). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Research Areas for CFTR ELISA Kit (NBP2-75258)

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Blogs on CFTR.

The ABCA1 Antibody and ABC Transporter Research
The ABC (ATP-binding cassette) transporters are a superfamily of transmembrane proteins which play an essential role in membrane transport in both prokaryotes and eukaryotes. In humans, ABC transporters have been implicated in many conditions. For exa...  Read full blog post.

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Bioinformatics

Gene Symbol CFTR