CFTR Antibody (13-1) [Unconjugated]

Images

 
CFTR was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CFTR R Domain Monoclonal Antibody (Catalog # MAB1660) at 15 µg/mL overnight at 4 °C. Before incubation with the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, ICC/IF
Clone
13-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

CFTR Antibody (13-1) [Unconjugated] Summary

Immunogen
beta -galactosidase-coupled CFTR
Cys590-Lys830 (predicted)
Accession # P13569
Specificity
Detects human CFTR. Specifically recognizes in vitro synthesized CFTR, recombinant CFTR protein, and non-recombinant CFTR protein (1, 2).
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CFTR
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 1-2 ug/10^6 cells
  • Immunofluorescence
Reviewed Applications
Read 2 Reviews rated 4
using
MAB1660 in the following applications:

Publications
Read Publications using
MAB1660 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a solution in PBS containing BSA.
*Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CFTR Antibody (13-1) [Unconjugated]

  • ABC35
  • ABCC7CF
  • ATP-binding cassette sub-family C member 7
  • ATP-binding cassette transporter sub-family C member 7
  • cAMP-dependent chloride channel
  • CFTR
  • CFTR/MRP
  • Channel conductance-controlling ATPase
  • cystic fibrosis transmembrane conductance regulator (ATP-binding cassettesub-family C, member 7)
  • cystic fibrosis transmembrane conductance regulator
  • cystic fibrosis transmembrane conductance regulator, ATP-binding cassette(sub-family C, member 7)
  • dJ760C5.1
  • EC 3.6.3
  • MRP7EC 3.6.3.49
  • TNR-CFTR

Background

Cystic fibrosis transmembrane conductance regulator (CFTR) is a multi-pass transmembrane protein that functions as a chloride channel. CFTR belongs to the ATP-binding cassette (ABC) superfamily. Mutations in CFTR cause the pulmonary disease, cystic fibrosis (CF). Specifically, deletion of phenyalanine at position 508 (DeltaF508-CFTR) results in a folding defect which impairs chloride channel function. The mechanism by which channel dysfunction relates to disease symptoms is a focus of intense research. CFTR dysfunction results in disruption of ion transport and subsequent blockage of airways by secreted mucus. CFTR may also play a role in the skeletal muscle atrophy and dysfunction that characterizes CF. In addition, CFTR-mediated chloride secretion underlies fluid accumulation and cyst growth in autosomal dominant polycystic kidney disease (ADPKD).
  1. Gregory, R.J. et al. (1990) Nature 347:328.
  2. Cheng, S.H. et al. (1990) Cell 63:827.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CFTR Antibody (MAB1660)(11)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Amphibian, Ovine, Xenopus.

We have publications tested in 6 applications: ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ICC
(4)
IHC
(1)
IHC-Fr
(2)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
Rat
(1)
Amphibian
(1)
Ovine
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using MAB1660 Applications Species
Z Bozoky, S Ahmadi, T Milman, TH Kim, K Du, M Di Paola, S Pasyk, R Pekhletski, JP Keller, CE Bear, JD Forman-Kay Synergy of cAMP and calcium signaling pathways in CFTR regulation Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28242698] (ICC, Human) ICC Human
Dong Q, Ernst S, Ostedgaard L, Shah V, Ver Heul A, Welsh M, Randak C Mutating the Conserved Q-loop Glutamine 1291 Selectively Disrupts Adenylate Kinase-dependent Channel Gating of the ATP-binding Cassette (ABC) Adenylate Kinase Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) and Reduces Channel Function in Primary Human Airway Epithelia. J Biol Chem, 2015;290(22):14140-53. 2015 [PMID: 25887396] (Western Blot, Human) Western Blot Human
Yan, Ziying, Keiser, Nicholas, Song, Yi, Deng, Xuefeng, Cheng, Fang, Qiu, Jianming, Engelhardt, John F A novel chimeric adenoassociated virus 2/human bocavirus 1 parvovirus vector efficiently transduces human airway epithelia. Mol Ther, 2013;21(12):2181-94. 2013 [PMID: 23896725] (IHC-Fr, Human) IHC-Fr Human
Sorio C, Buffelli M, Angiari C, Ettorre M, Johansson J, Vezzalini M, Viviani L, Ricciardi M, Verze G, Assael BM, Melotti P Defective CFTR expression and function are detectable in blood monocytes: development of a new blood test for cystic fibrosis. PLoS ONE, 2011;6(7):e22212. 2011 [PMID: 21811577] (Western Blot, Human) Western Blot Human
Wong AP, Keating A, Lu WY, Duchesneau P, Wang X, Sacher A, Hu J, Waddell TK Identification of a bone marrow-derived epithelial-like population capable of repopulating injured mouse airway epithelium. J. Clin. Invest., 2009;119(2):336-48. 2009 [PMID: 19164856] (ICC, Mouse) ICC Mouse
Davidson H, McLachlan G, Wilson A, Boyd AC, Doherty A, MacGregor G, Davies L, Painter HA, Coles R, Hyde SC, Gill DR, Amaral MD, Collie DD, Porteous DJ, Penque D Human-specific cystic fibrosis transmembrane conductance regulator antibodies detect in vivo gene transfer to ovine airways. Am. J. Respir. Cell Mol. Biol., 2006;35(1):72-83. 2006 [PMID: 16498081] (IHC-Fr, Ovine) IHC-Fr Ovine
Basavappa S, Vulapalli SR, Zhang H, Yule D, Coon S, Sundaram U Chloride channels in the small intestinal cell line IEC-18. J. Cell. Physiol., 2005;202(1):21-31. 2005 [PMID: 15389550] (ICC, Rat) ICC Rat
Koehler DR, Sajjan U, Chow YH, Martin B, Kent G, Tanswell AK, McKerlie C, Forstner JF, Hu J Protection of Cftr knockout mice from acute lung infection by a helper-dependent adenoviral vector expressing Cftr in airway epithelia. Proc. Natl. Acad. Sci. U.S.A., 2003;100(26):15364-9. 2003 [PMID: 14673110] (ICC, Human) ICC Human
Willumsen NJ, Amstrup J, Mobjerg N, Jespersen A, Kristensen P, Larsen EH Mitochondria-rich cells as experimental model in studies of epithelial chloride channels. Biochim. Biophys. Acta, 2002;1566(1):28-43. 2002 [PMID: 12421535] (IHC-P, Xenopus) IHC-P Xenopus
Bagorda A, Guerra L, Di Sole F, Hemle-Kolb C, Cardone RA, Fanelli T, Reshkin SJ, Gisler SM, Murer H, Casavola V Reciprocal protein kinase A regulatory interactions between cystic fibrosis transmembrane conductance regulator and Na+/H+ exchanger isoform 3 in a renal polarized epithelial cell model. J. Biol. Chem., 2002;277(24):21480-8. 2002 [PMID: 11937500] (Immunoprecipitation, Amphibian) Immunoprecipitation Amphibian
Show All 11 Publications.

Reviews for CFTR Antibody (MAB1660) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using MAB1660:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 02/10/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21811577
SpeciesHuman

Blocking

Blocking DetailsSee PMID 21811577

Details

Detection NotesSee PMID 21811577

Comments

CommentsIndependent review from antibodyresource.com
 
reviewed by:
Anonymous
WB Human 02/10/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 21952168
SpeciesHuman

Blocking

Blocking DetailsSee PMID 21952168

Details

Detection NotesSee PMID 21952168

Comments

CommentsIndependent review from antibodyresource.com

Product General Protocols

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FAQs for CFTR Antibody (MAB1660) (0)

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Bioinformatics Tool for CFTR Antibody (MAB1660)

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Blogs on CFTR.

The ABCA1 Antibody and ABC Transporter Research
The ABC (ATP-binding cassette) transporters are a superfamily of transmembrane proteins which play an essential role in membrane transport in both prokaryotes and eukaryotes. In humans, ABC transporters have been implicated in many conditions. For exa...  Read full blog post.

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Anonymous
02/10/2015
Application: WB
Species: Human

 
Anonymous
02/10/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CFTR
Uniprot