CEBP gamma Antibody

Immunohistochemistry-Paraffin: CEBP gamma Antibody [NBP1-36944] - (2.5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CEBP gamma Antibody Summary

• Description: Novus Biologicals Goat CEBP gamma Antibody (NBP1-36944) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-CEBP gamma Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-ENTTADGDNAGQ corresponding to C-Terminus according to NP_001797.1.
• Epitope: C Terminus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CEBPG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 1 ug/ml
• Application Notes: Western blot: Preliminary experiments gave an approx. 26kDa band in Human Lung lysates and in lysates of cell lines A549 after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 16.4kDa according to NP_001797.1. The 26kDa band was successfully blocked by incubation with the immunizing peptide.
  IHC: Paraffin embedded Human Brain (Cortex).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CEBP gamma Antibody

• c/EBP gamma
• CCAAT/enhancer binding protein (C/EBP), gamma
• CCAAT/enhancer-binding protein gamma
• GPE1BP
• IG/EBP-1

Background

The transcription factor C/EBP alpha (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP alpha appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP beta, C/EBP gamma,C/EBP delta and C/EBP episilon, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha. Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CEBP gamma Antibody (NBP1-36944)(1)

Publication using NBP1-36944

• Mullins DN, Crawford EL, Khuder SA et al. CEBPG transcription factor correlates with antioxidant and DNA repair genes in normal bronchial epithelial cells but not in individuals with bronchogenic carcinoma. BMC Cancer 2005-10-29 [PMID: 16255782]

Bioinformatics

• Gene Symbol: CEBPG
• Entrez:
  • 1054(Human)
• Uniprot:
  • NP_001797.1(Human)