CEACAM1/CD66a Antibody (723629) [Unconjugated]

Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.1 µg/mL of Rat Anti-Mouse CEACAM-1/CD66a Monoclonal Antibody (Catalog # MAB6480) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CEACAM-1/CD66a at approximately 110-120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Mouse splenocytes were stained with Rat Anti-Mouse CEACAM‑1/CD66a Monoclonal Antibody (Catalog # MAB6480) followed by Fluorescein-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0104B) and Rat Anti-Mouse B220/CD45R Allophycocyanin-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # MAB005).

CEACAM‑1/CD66a was detected in perfusion fixed frozen sections of mouse liver using Rat Anti-Mouse CEACAM‑1/CD66a Monoclonal Antibody (Catalog # MAB6480) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to bile canaliculi. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: 723629
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CEACAM1/CD66a Antibody (723629) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CEACAM-1/CD66a
  Glu35-Gly428
  Accession # P31809
• Specificity: Detects mouse CEACAM-1/CD66a in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CECAM-1, -3, -5, or -6 is observed. In Western blots, no cross-reactivity with rhCEACAM-1, -3, -4, -5, -6, or -7 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: Ceacam1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEACAM1/CD66a Antibody (723629) [Unconjugated]

• antigen CD66
• BGP1
• BGP-1
• BGP1BGPBiliary glycoprotein 1
• BGPI
• Biliary Glycoprotein 1
• biliary glycoprotein adhesion molecule
• carcinoembryonic antigen-related cell adhesion molecule 1 (biliaryglycoprotein)
• carcinoembryonic antigen-related cell adhesion molecule 1
• CD66a antigen
• CD66a
• Cea-1
• CEACAM1
• CEACAM-1
• Hv-1
• Hv-2
• MHVR

Background

CEACAM-1 (Carcinoembryonic antigen-related cell adhesion molecule 1; also BGP-1, CD66a and MHVR1) is a 110-120 kDa member of the CEACAM subfamily, CEA family of proteins. It has a wide expression pattern, being found on neutrophils, dendritic cells, endothelial cells, colonic epithelium and hepatocytes. It mediates cell adhesion, and appears to regulate insulin levels and signaling by interacting with the insulin receptor. It also demonstrates proangiogenic effects by inducing endothelial cells to proliferate and form capillary-like tubules. Finally, CEACAM-1 is a known receptor for mouse hepatitis virus. Mature mouse CEACAM-1 is a 487 amino acid (aa) type I transmembrane glycoprotein. Its contains a 394 aa extracellular region (aa 35-428) that shows one V-type (aa 35-142) and three C2-type (aa 147-411) Ig-like domains, plus a 74 aa cytoplasmic domain. Three alternate splice forms exist. One contains a four aa substitution for aa 455-521, a second shows a Gln substitution for aa 142-322, and a third possesses a combination of the first two splice patterns. CEACAM-1 forms homodimers. Over aa 35-428, mouse CEACAM-1 shares 56% and 70% aa identity with human and rat CEACAM-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Ceacam1
• Uniprot:
  • P31809()