CEACAM1/CD66a Antibody (283340) [Allophycocyanin]

• Applications: Flow
• Clone: 283340
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

CEACAM1/CD66a Antibody (283340) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CEACAM‑1/CD66a
  Gln35-Gly428
  Accession # P13688
• Specificity: Detects human CEACAM‑1/CD66a in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) CD31, rhICAM-1, -2, -3, recombinant mouse MAdCAM-1, or rhVCAM-1.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEACAM1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Carcinoembryonic Antigen (CEA)-related Cell Adhesion Molecule 1 (CEACAM-1), also known as BGP-1, is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily (1-3). It is one of seven human CEACAM subfamily genes that are essentially divided equally between type I transmembrane proteins (CEACAM-1, 3, and 4) and GPI-linked molecules (CEACAM-5-8). There is no CEACAM-2 in human. The gene for human CEACAM-1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74 aa cytoplasmic region (4, 5). The ECD contains one N-terminal V-type Ig-like domain, followed by three C2-type Ig-like domains. It shows considerable glycosylation, including high mannose residues and (sialyl) Lewis^X (1). The cytoplasmic region shows one ITIM motif and a calmodulin binding site (1-3). In addition to the full length form, ten alternate splice forms have been reported (1, 4, 6-8). There are three soluble and seven transmembrane isoforms, with variations occurring in both the ECD and cytoplasmic region. All ten alternate splice forms contain the V-type Ig-like domain (aa’s 35-142). The three soluble forms also contain the first two C2-type Ig-like domains (aa’s 145-317), with differences coming in the third C2-type Ig-like domain (6). The seven transmembrane isoforms are highly divergent. Five of the seven contain the V-type plus the first two C2-type domains and then diverge considerably both in the ECD and cytoplasmic region. The remaining two contain only the V‑type Ig-like domain, the transmembrane region, and either a full-length or truncated cytoplasmic tail (1, 8). The actual functions of the isoforms are unclear. Full-length mouse and rat CEACAM-1 are approximately 57% aa identical to human CEACAM-1; in the V‑type Ig-like domain, they are 58% and 56% aa identical, respectively. The full-length molecule is found on neutrophils, bile duct epithelium, activated NK cells, colonic columnar epithelium and endothelium. It is known to act as an intercellular adhesion molecule, forming both homotypic, and heterotypic bonds with CEA and CEACAM-6/NCA (3, 9). On neutrophils, CEACAM-1 also binds to dendritic cell CD-SIGN via its Le^X moiety, inducing dendritic cell maturation and a subsequent Th1-type response (10,11).

1. Beauchemin, N. et al. (1999) Exp. Cell Res. 252:243.
2. Thompson, J. et al. (1992) Genomics 12:761.
3. Waggener, C. and S. Ergun (2000) Exp. Cell Res. 261:19.
4. Barnett, T.R. et al. (1989) J. Cell Biol. 108:267.
5. Hinoda, Y. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6959.
6. Kuroki, M. et al. (1991) Biochem. Biophys. Res. Commun. 176:578.
7. Barnett, T.R. et al. (1993) Mol. Cell. Biol. 13:1273.
8. Watt, S.M. et al. (1994) Blood 84:200.
9. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.
10. Klaas, P.J.M. et al. (2005) FEBS Lett. 579:6159.
11. Bogoevska, V. et al. (2005) Glycobiology 16:197.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CEACAM1