CDYL Antibody

Western Blot: CDYL Antibody [NB300-874] - (1ug/ml) of Human Testes lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CDYL Antibody Summary

• Immunogen: Peptide with sequence C-SMLKYLQRKIDEF corresponding to C-Terminus according to NP_004815.3, NP_001137442.1, NP_001137443.1.
• Specificity: This antibody is expected to recognize both eported isoforms NP_004815.3; NP_001137442.1; NP_001137443.1). Variants (NP_001137442.1; NP_001137443.1) encode the same isoform.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDYL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in human testis lysates is reported in scientific literature (PMID: 12072557). An additional band of unknown identity was also consistently observed at 26 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDYL Antibody

• CDYL1bA620A17.2 (chromodomain protein, Y chromosome-like)
• CDY-like
• CDY-like, autosomal
• chromodomain protein, Y chromosome-like
• chromodomain protein, Y-like
• chromodomain Y-like protein
• DKFZP586C1622
• EC 2.3.1.48
• MGC131936
• testis-specific chromodomain Y-like protein

Background

Chromodomain Y is a primate-specific Y-chromosomal gene family expressed exclusively in the testis and implicated in infertility. Although the Y-linked genes are testis-specific, this autosomal gene is ubiquitously expressed. The Y-linked genes arose by retrotransposition of an mRNA from this gene, followed by amplification of the retroposed gene. Proteins encoded by this gene superfamily possess a chromodomain, a motif implicated in chromatin binding and gene suppression, and a catalytic domain believed to be involved in histone acetylation. Multiple proteins are encoded by transcript variants of this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDYL Antibody (NB300-874)(2)

Publications using NB300-874

• Lahn BT, Tang ZL, Zhou J et al. Previously uncharacterized histone acetyltransferases implicated in mammalian spermatogenesis. Proc Natl Acad Sci USA 2002-06-01 [PMID: 12072557]
• Lahn BT, Page DC. Retroposition of autosomal mRNA yielded testis-specific gene family on human Y chromosome. Nat Genet 1999-04-01 [PMID: 10192397]

Bioinformatics

• Gene Symbol: CDYL
• Entrez:
  • 9425(Human)
  • 12593(Mouse)
• Uniprot:
  • NP_004815(Human)