CDK5RAP1 Overexpression Lysate (Native)


Western Blot: CDK5RAP1 Overexpression Lysate (Adult Normal) [NBL1-09042] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CDK5RAP1.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

CDK5RAP1 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC216600 and based on accession number NM_016408. The protein contains a C-terminal DDK tag.
Homo sapiens CDK5 regulatory subunit associated protein 1 (CDK5RAP1), transcript variant 1, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for CDK5RAP1

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CDK5RAP1 Overexpression Lysate (Native)

  • C20orf34
  • C42
  • CDK5 activator-binding protein C42
  • CDK5 regulatory subunit associated protein 1
  • CDK5 regulatory subunit-associated protein 1
  • CDK5RAP1.3
  • CDK5RAP1.4
  • CGI-05
  • chromosome 20 open reading frame 34
  • HSPC167


Neuronal CDC2-like kinase, which is involved in the regulation of neuronal differentiation, is composed of a catalytic subunit, CDK5, and an activating subunit, p25NCK5A. The protein encoded by this gene binds to p25NCK5A and therefore may be involved in neuronal differentiation. Multiple transcript variants exist for this gene, but the full-length natures of only two have been determined. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Gene Symbol CDK5RAP1