Mouse CDK4 ELISA Kit (Colorimetric)

ELISA: Mouse CDK4 ELISA Kit (Colorimetric) [NBP2-75172] - Samples were spiked with high concentrations of Mouse CDK4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse CDK4 ELISA Kit (Colorimetric) [NBP2-75172] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 62.50 - 4000 pg/mL
• Sensitivity: 37.50 pg/mL

Mouse CDK4 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse CDK4 in samples. No significant cross-reactivity or interference between Mouse CDK4 and analogues was observed.
• Standard Curve Range: 62.50 - 4000 pg/mL
• Sensitivity: 37.50 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.79%
• Intra-Assay: CV% < 4.72%
• Spike Recovery: 88-104%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CDK4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse CDK4 ELISA Kit (Colorimetric)

• CDK4
• Cell division protein kinase 4
• CMM3
• cyclin-dependent kinase 4
• EC 2.7.11
• EC 2.7.11.22
• melanoma cutaneous malignant, 3
• MGC14458
• PSK-J3
• PSK-J3cell division kinase 4

Background

Cdk4 is encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CDK4