CDK4 Antibody (SD20-42)

Images

 
Western Blot: CDK4 Antibody (SD20-42) [NBP2-67736] - Western blot analysis of CDK4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The ...read more
Immunocytochemistry/ Immunofluorescence: CDK4 Antibody (SD20-42) [NBP2-67736] - Staining Cdk4 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: CDK4 Antibody (SD20-42) [NBP2-67736] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-Cdk4 antibody. Counter stained with hematoxylin.
Flow Cytometry: CDK4 Antibody (SD20-42) [NBP2-67736] - Analysis of MCF-7 cells with Cdk4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa ...read more
Western Blot: CDK4 Antibody (SD20-42) [NBP2-67736] - Analysis of Cdk4 on Hela cells lysates using anti-Cdk4 antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: CDK4 Antibody (SD20-42) [NBP2-67736] - Staining Cdk4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: CDK4 Antibody (SD20-42) [NBP2-67736] - Staining Cdk4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunohistochemistry-Paraffin: CDK4 Antibody (SD20-42) [NBP2-67736] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cdk4 antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
SD20-42
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CDK4 Antibody (SD20-42) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within C-terminal human Cdk4. (SwissProt: P11802 Human ;SwissProt: P30285 Mouse ;SwissProt: P35426 Rat)
Localization
Nucleus, Nucleus membrane, Cytoplasm.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CDK4
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CDK4 Antibody (SD20-42)

  • CDK4
  • Cell division protein kinase 4
  • CMM3
  • cyclin-dependent kinase 4
  • EC 2.7.11
  • EC 2.7.11.22
  • melanoma cutaneous malignant, 3
  • MGC14458
  • PSK-J3
  • PSK-J3cell division kinase 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CDK4