CDK20 Antibody

Western Blot: CDK20 Antibody [NBP1-32415] - Sample(30 ug whole cell lysate)A:Hep G2 12% SDS PAGE, antibody diluted at 1:500.

Immunocytochemistry/Immunofluorescence: CDK20 Antibody [NBP1-32415] - Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CCRK protein stained by CCRK antibody diluted at 1:500. Blue: Hoechst 33343 staining.

Product Discontinued

CDK20 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human CCRK. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (93%), Rat (92%), Porcine (92%), Bovine (91%), Primate (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDK20
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (98%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for CDK20 Antibody

• CAK-kinase p42
• CCRK
• CCRKEC 2.7.11.22
• CDCH
• CDCHP42
• CDK20
• CDK-activating kinase p42
• cell cycle related kinase
• Cell cycle-related kinase
• Cell division protein kinase 20
• cyclin-dependent kinase 20
• Cyclin-dependent protein kinase H
• Cyclin-kinase-activating kinase p42
• EC 2.7.11
• p42
• PNQALRE

Background

The protein encoded by this gene contains a kinase domain most closely related to the cyclin-dependent protein kinases. The encoded kinase activates CDK2 and is involved in cell growth. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for CDK20 Antibody (NBP1-32415). (Showing 1 - 1 of 1 FAQ).

1. Can this antibody detect isoform 1 of CDK20? The exact epitope is not indicate in datasheet.
   • Yes, this antibody should detect Isoform 1. The antigen share considerable homology with Isoform 2 100%, Isoform 3 - 92% and Isoform 4 - 89% as well. This antibody been tested to recognize human CDK20 in our lab and at this time we have not performed further specificity testing against different isoforms

Bioinformatics

• Gene Symbol: CDK20