CDIPT Antibody

Immunocytochemistry/Immunofluorescence: CDIPT Antibody [NBP2-49233] - Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm, nuclear membrane & plasma membrane. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: CDIPT Antibody [NBP2-49233] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: CDIPT Antibody [NBP2-49233] - Staining of human cerebellum shows strong granular cytoplasmic positivity in purkinje cells.

Immunohistochemistry-Paraffin: CDIPT Antibody [NBP2-49233] - Staining of human testis shows moderate granular cytoplasmic positivity in Leydig cells.

Immunohistochemistry-Paraffin: CDIPT Antibody [NBP2-49233] - Staining of human liver shows weak cytoplasmic positivity in hepatocytes.

Product Discontinued

CDIPT Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: LTASSFYLLSGLLDAFDGHAARALNQGTRFGAMLDMLTDR
• Predicted Species: Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDIPT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CDIPT Antibody

• CDP-diacylglycerol--inositol 3-phosphatidyltransferase
• EC 2.7.8.11
• Phosphatidylinositol synthase
• PI synthase
• PIS1MGC1328
• PISCDP-diacylglycerol--inositol 3-phosphatidyltransferase (phosphatidylinositolsynthase)
• PtdIns synthase

Background

Phosphatidylinositol breakdown products are ubiquitous second messengers that function downstream of many Gprotein-coupled receptors and tyrosine kinases regulating cell growth, calcium metabolism, and protein kinase Cactivity. Two enzymes, CDP-diacylglycerol synthase and phosphatidylinositol synthase, are involved in the biosynthesisof phosphatidylinositol. Phosphatidylinositol synthase, a member of the CDP-alcohol phosphatidyl transferase class-Ifamily, is an integral membrane protein found on the cytoplasmic side of the endoplasmic reticulum and the Golgiapparatus. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDIPT