CDCP1 Antibody (B2-E6)

Western Blot: CDCP1 Antibody (B2-E6) [NBP2-75929] - analysis of CDCP1 on K562 cell lysates using anti-CDCP1 antibody at 1/200 dilution.

Immunocytochemistry/Immunofluorescence: CDCP1 Antibody (B2-E6) [NBP2-75929] - staining CDCP1 (red) in A549 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CDCP1 Antibody (B2-E6) [NBP2-75929] - Analysis of mouse colon tissue using anti-CDCP1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: CDCP1 Antibody (B2-E6) [NBP2-75929] - staining CDCP1 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CDCP1 Antibody (B2-E6) [NBP2-75929] - Analysis of rat skeletal muscle tissue using anti-CDCP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CDCP1 Antibody (B2-E6) [NBP2-75929] - Analysis of human tonsil tissue using anti-CDCP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CDCP1 Antibody (B2-E6) [NBP2-75929] - Analysis of human breast cancer tissue using anti-CDCP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CDCP1 Antibody (B2-E6) [NBP2-75929] - Analysis of human colon cancer tissue using anti-CDCP1 antibody. Counter stained with hematoxylin.

Product Discontinued

CDCP1 Antibody (B2-E6) Summary

• Immunogen: Recombinant protein within Human CDCP1 aa 487-836 / 836. (SwissProt: Q9H5V8 Human; SwissProt: Q5U462 Mouse)
• Localization: Cell membrane. Secreted.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDCP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDCP1 Antibody (B2-E6)

• CD318 antigen
• CD318
• CDCP1
• CUB domain containing protein 1
• CUB domain-containing protein 1
• Membrane glycoprotein gp140
• SIMA135
• SIMA135TRASK
• Subtractive immunization M plus HEp3-associated 135 kDa protein
• Transmembrane and associated with src kinases

Background

CDCP1 is encoded by this gene is a transmembrane protein containing three extracellular CUB domains. This protein is found to be overexpressed in colon and lung cancers. Its expression level is correlated with the metastatic ability of carcinoma cells. This protein is located on the cell surface. It has been shown to be tyrosine phosphorylated in a cancer cell line. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDCP1