CDCP1 Antibody (469114) [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clone: 469114
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CDCP1 Antibody (469114) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CDCP1
  Arg25-Ala667
  Accession # Q5U462
• Specificity: Detects mouse CDCP1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CDCP1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: CDCP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDCP1 Antibody (469114) [Unconjugated]

• CD318 antigen
• CD318
• CDCP1
• CUB domain containing protein 1
• CUB domain-containing protein 1
• Membrane glycoprotein gp140
• SIMA135
• SIMA135TRASK
• Subtractive immunization M plus HEp3-associated 135 kDa protein
• Transmembrane and associated with src kinases

Background

CUB domain containing protein 1 (CDCP1), also known as SIMA135, is a 140 kDa type I transmembrane glycoprotein with three CUB protein‑protein interaction domains in its 635 aa extracellular region. The 148 aa cytoplasmic region contains canonical phosphorylation sites for Src kinase family members and binding sites for SH3 domains. By alternative splicing, a secreted 310 aa residue form of CDCP1 also exists. The amino-terminal region of approximately 265 aa of the type I membrane CDCP1 can also be proteolytically cleaved. CDCP1 is found on the surface of epithelial and bone marrow-derived stem cells. The extracellular region of human CDCP1 shares 84% aa identity with that of the mouse protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDCP1
• Uniprot:
  • Q5U462()