CDC42EP2 Antibody (2H7) - Azide and BSA Free

Western Blot: CDC42EP2 Antibody (2H7) [H00010435-M01] - Analysis of CDC42EP2 expression in transfected 293T cell line by CDC42EP2 monoclonal antibody (M01), clone 2H7.Lane 1: CDC42EP2 transfected lysate(22.5 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 2H7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CDC42EP2 Antibody (2H7) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CDC42EP2 Antibody (2H7) - Azide and BSA Free (H00010435-M01) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-CDC42EP2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: CDC42EP2 (NP_006770, 102 a.a. ~ 210 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLLKNAISLPVIGGPQALTLPTAQAPPKPPRLHLETPQPSPQEGGSVDIWRIPETGSPNSGLTPESGAEEPFLSNASSLLSLHVDLGPSILDDVLQIMDQDLDSMQIPT
• Specificity: CDC42EP2 - CDC42 effector protein (Rho GTPase binding) 2
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDC42EP2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CDC42EP2 Antibody (2H7) - Azide and BSA Free

• BORG1CEP2Binder of Rho GTPases 1
• CDC42 effector protein (Rho GTPase binding) 2
• cdc42 effector protein 2
• CRIB-containing BOGR1 protein

Background

CDC42, a small Rho GTPase, regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to, and negatively regulate the function of, CDC42. Coexpression of this protein with dominant negative mutant CDC42 protein in fibroblast was found to induce pseudopodia formation, which suggested a role of this protein in actin filament assembly and cell shape control.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDC42EP2 Antibody (H00010435-M01)(1)

Publication using H00010435-M01

• Schnack C, Danzer KM, Hengerer B et al. Protein array analysis of oligomerization-induced changes in alpha-synuclein protein-protein interactions points to an interference with CDC42 effector proteins. Neuroscience 2008-02-01 [PMID: 18541383]

Bioinformatics

• Gene Symbol: CDC42EP2
• Entrez:
  • 10435(Human)
• OMIM:
  • 606132(Human)
• Uniprot:
  • NP_006770(Human)