CDC40 Antibody (SD085-9)

Western Blot: CDC40 Antibody (SD085-9) [NBP2-67819] - Analysis of CDC40 on Hela cells lysates using anti-CDC40 antibody at 1/2,000 dilution.

Immunocytochemistry/Immunofluorescence: CDC40 Antibody (SD085-9) [NBP2-67819] - Staining CDC40 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CDC40 Antibody (SD085-9) [NBP2-67819] - Analysis of paraffin-embedded rat brain tissue using anti-CDC40 antibody. Counter stained with hematoxylin.

Flow Cytometry: CDC40 Antibody (SD085-9) [NBP2-67819] - Analysis of K562 cells with CDC40 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: CDC40 Antibody (SD085-9) [NBP2-67819] - Staining CDC40 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CDC40 Antibody (SD085-9) [NBP2-67819] - Staining CDC40 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CDC40 Antibody (SD085-9) [NBP2-67819] - Analysis of paraffin-embedded mouse brain tissue using anti-CDC40 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CDC40 Antibody (SD085-9) [NBP2-67819] - Analysis of paraffin-embedded mouse colon tissue using anti-CDC40 antibody. Counter stained with hematoxylin.

Product Discontinued

CDC40 Antibody (SD085-9) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human CDC40 aa 41-90 / 579. (SwissProt: O60508 Human; SwissProt: Q9DC48 Mouse; Entrez Gene: 361859 Rat)
• Localization: Nucleus, Nucleus speckle.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDC40
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CDC40 Antibody (SD085-9)

• cell division cycle 40 homolog (S. cerevisiae)
• cell division cycle 40 homolog (yeast)
• Cell division cycle 40 homolog
• Ehb3
• EH-binding protein 3
• hPRP17
• MGC102802
• pre-mRNA splicing factor 17
• pre-mRNA-processing factor 17
• PRP17 homolog
• PRP17EHB3PRPF17FLJ10564

Background

Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp17 protein, which functions in two different cellular processes: pre-mRNA splicing and cell cycle progression. It suggests that this protein may play a role in cell cycle progression. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC40